Pharmacokinetic analysis of site-directed and light-sensitive liposome

定点光敏脂质体的药代动力学分析

• 批准号: 23592604
• 负责人: HASHIDA Noriyasu
• 金额: $ 3.33万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592604/
• 关键词: 眼免疫学; リポソーム; ドラッグデリバリーシステム; 炎症; ぶどう膜炎; 光感受性; ドラッグデリバリー; ベルテポルフィン

The site-directed and light-sensitive liposome (SDLSL) was prepared using a light-sensitive substance verteporfin in this study. SDLSL could be effectively destroyed by laser irradiation of 532nm and 698nm. We also encapsulated the cisplatin (CDDP), an anticancer agent, into SDLSL in order to investigate further to check the release of CDDP by laser irradiation. As a result, we observed the release of anticancer agent. We validated tumor-suppressive efficacy of the CDDP encapsulating SDLSL in vitro and in vivo. Using colon 26 cell line and CDDP encapsulating SDLSL, strong cancer cell growth inhibitory effect was obtained with light irradiation group in vitro and in vivo, respectively. From these results, SDLSL can be a highly efficacious site-directed drug delivery system in vitro and in vivo. Using SDLSL as a vehicle for drug delivery, substantial pharmacologic effects with minimum side effects into site-directed tissues should be achieved.

本研究以光敏物质维替泊芬为原料，制备了定点光敏脂质体（SDLSL）。532nm和698nm的激光辐照能有效地破坏SDLSL。我们还将抗癌药物顺铂（CDDP）包裹到SDLSL中，以进一步研究激光照射对CDDP释放的影响。结果，我们观察到抗癌剂的释放。我们在体外和体内验证了包封SDLSL的CDDP的肿瘤抑制功效。以结肠癌细胞株Colon26为研究对象，光照射组在体外和体内均能明显抑制肿瘤细胞的生长。从这些结果，SDLSL可以是一个非常有效的定点药物输送系统在体外和体内。使用SDLSL作为药物递送的媒介物，应当实现对位点定向组织的实质性药理作用和最小副作用。

项目成果

High-efficacy site-directed drug delivery system using sialyl-Lewis X conjugated liposome

• DOI: 10.1016/j.exer.2007.10.004
• 发表时间: 2008-01-01
• 期刊: EXPERIMENTAL EYE RESEARCH
• 影响因子: 3.4
• 作者: Hashida, Noriyasu;Ohguro, Nobuyuki;Tano, Yasuo
• 通讯作者: Tano, Yasuo

遺伝子疾患Mook 23臨床・創薬利用が見えてきたmicroRNA 11.眼科領域におけるmiRNA

遗传病 Mook 23 microRNA 的临床和药物发现应用 11. 眼科领域的 miRNA

• 发表时间: 2012
• 作者: Ooto S;Hangai M;Tomidokoro A;Saito H;Araie M;Otani T;Kishi S;Matsushita K;Maeda N;Shirakashi M;Abe H;Ohkubo S;Sugiyama K;Iwase A;Yoshimura N;橋田徳康
• 通讯作者: 橋田徳康

Sustained delivery of a HIF-1 antagonist for ocular neovascularization.

• DOI: 10.1016/j.jconrel.2013.10.008
• 发表时间: 2013-12-28
• 期刊: Journal of controlled release : official journal of the Controlled Release Society
• 作者: Iwase T;Fu J;Yoshida T;Muramatsu D;Miki A;Hashida N;Lu L;Oveson B;Lima e Silva R;Seidel C;Yang M;Connelly S;Shen J;Han B;Wu M;Semenza GL;Hanes J;Campochiaro PA
• 通讯作者: Campochiaro PA

EXPRESSION ANALYSIS OF MICRORNA DURING DEVELOPMENT OF MURINE EXPERIMENTAL AUTOIMMUNE UVEORETINITIS.

小鼠实验性自身免疫性葡萄膜视网膜炎发生过程中微小RNA的表达分析。

• 发表时间: 2011
• 作者: Hashida N;Nishida K.
• 通讯作者: Nishida K.

Anterior segment optical coherence tomography findings of presumed intraocular tuberculosis

• DOI: 10.3109/15569527.2010.517231
• 发表时间: 2011-03-01
• 期刊: CUTANEOUS AND OCULAR TOXICOLOGY
• 影响因子: 1.6
• 作者: Hashida, Noriyasu;Terubayashi, Aya;Ohguro, Nobuyuki
• 通讯作者: Ohguro, Nobuyuki