Tri-functional antibody to OSCC and complement regulatory proteins

针对 OSCC 和补体调节蛋白的三功能抗体

• 批准号: 21791828
• 负责人: IMAI Masaki
• 金额: $ 2.75万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791828/
• 关键词: 口腔癌; 抗体; 免疫療法; 補体

We have studied the anti-tumor activities of an anti-MUC1 antibody with oral squamous cell carcinoma (OSCC). We hypothesized the inhibition of complement regulatory proteins (CRPs) could enhance anti-tumor activity. Therefore, that reversing the effects of complement regulatory proteins expressed on a tumor cell surface will allow more effective immune-mediated clearance of tumor cells and improve prospects for successful immunotherapy. When the complement-dependent cytotoxicity was compared treatment with both an anti-MUC1 mAb and inhibiting mAbs of CD55 or CD59 to an anti-MUC1 mAb alone, a 2- to 3-fold increase in C-dependent cell lysis was observed, respectively. It was also proposed to produce the tri-functional antibodies recognized both MUC1 and CRPs. We done to produce the expression vectors of tri-functional antibodies.

我们已经研究了抗MUC1抗体与口腔鳞状细胞癌（OSCC）的抗肿瘤活性。我们假设抑制补体调节蛋白（CRP）可以增强抗肿瘤活性。因此，逆转肿瘤细胞表面上表达的补体调节蛋白的作用将允许更有效的免疫介导的肿瘤细胞清除，并改善成功免疫治疗的前景。当将用抗MUC1 mAb和抑制性CD55或CD59 mAb两者处理的补体依赖性细胞毒性与单独的抗MUC1 mAb处理进行比较时，分别观察到C依赖性细胞溶解增加2至3倍。还提出了产生识别MUC1和CRP的三功能抗体。本研究成功制备了三功能抗体的表达载体。

项目成果

Procarboxypeptidase R Deficiency Causes Increased Lethality in Concanavalin A-Induced Hepatitis in Female Mice

• DOI: 10.1248/bpb.33.1256
• 发表时间: 2010-07-01
• 期刊: BIOLOGICAL & PHARMACEUTICAL BULLETIN
• 影响因子: 2
• 作者: Asai, Suzuka;Kimbara, Noriaki;Okada, Noriko
• 通讯作者: Okada, Noriko

新型H1N1インフルエンザの重症化における補体アナフィラトキシンの関与

补体过敏毒素与新型 H1N1 流感严重程度的关系

• 发表时间: 2010
• 作者: 太田里永子;伊藤嘉規;鳥居ゆか;木村宏;岡田則子,今井優樹
• 通讯作者: 岡田則子,今井優樹

腫瘍細胞上の補体制御因子によるT細胞応答の制御.

补体调节剂对肿瘤细胞的 T 细胞反应的调节。

• 发表时间: 2009
• 作者: 今井優樹;Varela JC;Atkinson C;太田里永子;岡田則子;Rapisardo M;Tomlinson S;Imai M;太田里永子;Husseim MH;今井優樹
• 通讯作者: 今井優樹

Endothelin receptor antagonist attenuates inflammatory response and prolongs the survival time in a neonatal sepsis model

• DOI: 10.1007/s00134-010-2040-0
• 发表时间: 2010-12-01
• 期刊: INTENSIVE CARE MEDICINE
• 影响因子: 38.9
• 作者: Goto, Tatenobu;Hussein, Mohamed Hamed;Okada, Hidechika
• 通讯作者: Okada, Hidechika

An acetlated anti-C5a complementary peptide reduced cytokines and free radicals and prolongs survival time in a neonatal sepsis model.

乙酰化抗 C5a 互补肽可减少新生儿败血症模型中的细胞因子和自由基，并延长存活时间。

• 发表时间: 2009
• 作者: 今井優樹;Varela JC;Atkinson C;太田里永子;岡田則子;Rapisardo M;Tomlinson S;Imai M;太田里永子;Husseim MH
• 通讯作者: Husseim MH