The development of cell spheroid implantation therapy using the peripheral blood and the closed culture system

众所周知，间充质细胞凝聚在膜性成骨的早期阶段起着重要的作用，三维和动态的流动可以积极促进骨分化。在本研究中，我们尝试在不使用任何合成材料或外源生物材料的情况下进行骨组织工程。为了模拟骨发育，我们将骨髓基质细胞和外周血单核细胞通过旋转培养生成三维细胞球体，并将其植入裸鼠颅骨缺损。采用组织学、免疫化学、显微CT、拉曼光谱和力学分析等方法对大鼠的骨再生能力进行评价。

It is well known that mesenchymal cell condensation plays an important role in the early stages of membranous osteogenesis, three-dimensional and dynamic flow can positively enhance the osteodifferentiation. In this study, we tried to conduct bone tissue engineering without any synthetic or exogenous biomaterials. To imitate bone development, we generated three-dimensional cellular spheroid using bone marrow derived stromal cells and peripheral blood mononuclear cells by rotation culture, then implanted them into the nude rat calvarial defects. We evaluated the bone regenerative capacity of the rats by histological, immunochemical, maicro CT, raman spectrum analysis and mechanical analysis.