The study of oral cancer diagnosis by p53 mutation gene detection using saliva

唾液p53突变基因诊断口腔癌的研究

• 批准号: 21791993
• 负责人: KOMATSUBARA Hideki
• 金额: $ 2.66万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791993/
• 关键词: 口腔癌; 唾液; 転移; 歯学

Oral cavity is the entrance of the gastrointestinal tract is a site with high visibility. Meanwhile, the inflammatory disease is associated with the occurrence of teeth ranging and malignant disease, the differentiation is difficult. In order to confirm the diagnosis of cancer is a biopsy is needed. Dissemination of cancer cells by the surgical stress, could be said to promote metastasis, early detection is necessary to improve the prognosis of non-invasive diagnostic procedure. Collected over time can be tried basic research may be available as a specific tumor marker of saliva can be collected non-invasively. Oral cancer cell lines derived from mouse submandibular gland was removed HSC-transplanted orthotopically 3. At the same time of HSC-3 p53 gene mutation sites were identified, PCR-specific primers were required. Confirmed to have similar sensitivity to confirm the sensitivity of β-globin primers derived from known this.

口腔是胃肠道的入口，是一个能见度很高的部位。同时，炎症性疾病与牙齿范围及恶性疾病的发生有关，难以鉴别。为了确认癌症的诊断，需要进行活组织检查。肿瘤细胞的播散受手术应激的影响，可以说促进了转移，早期发现是改善预后的无创诊断程序所必需的。经过一段时间的收集，可以进行基础研究，因为唾液的特定肿瘤标志物可以非侵入性地收集。从小鼠颌下腺中提取口腔癌细胞系，采用hsc原位移植3。在确定HSC-3 p53基因突变位点的同时，需要pcr特异性引物。证实具有相似的敏感性，证实敏感性的β-珠蛋白引物来源于此。

项目成果

3D-CTangiographyにより診断しえた顔面静脈切断による口腔内巨大血腫の1例

3D-CT血管造影诊断面静脉截肢所致口内巨大血肿一例

• 发表时间: 2010
• 作者: Kato K;Kawashiri S;Yoshizawa K;Kitahara H;Okamune A;Sugiura S;Noguchi N;Yamamoto E;小松原秀紀
• 通讯作者: 小松原秀紀

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