Establishment of human cells as feeder cells of human embryonic stem cells

与小鼠胚胎干细胞相比，人类胚胎干细胞难以在体外培养。相比之下，与人ES细胞相比，人胚胎癌(EC)细胞更容易培养。在这项研究中，我们评估了人类 EC 细胞系，以研究哪些人类细胞可以用作饲养细胞。我们在人类胚胎干细胞的维持和多能性的保存方面设定了人类饲养细胞的规格。我们确定了能够成为饲养细胞的人类细胞类型，并确实建立了人类饲养细胞。

Human embryonic stem(ES) cells are difficult to be cultivated in vitro, compared with murine ES cells. In contrast, human embryonal carcinoma(EC) cells can easily be cultivated, compared with human ES cells. In this study, we evaluated human EC cell lines to investigate which human cells can be used as feeder cells. We set specification of human feeder cells in terms of maintenance of human ES cells and preservation of pluripotency. We determined human cell types that are capable of being feeder cells and indeed established human feeder cells.