Establishment of human cells as feeder cells of human embryonic stem cells

建立人类细胞作为人类胚胎干细胞的饲养细胞

• 批准号: 21659092
• 负责人: UMEZAWA Akihiro
• 金额: $ 2.15万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659092/
• 关键词: 成育医学; ES細胞; フィーダー細胞; 糖鎖; 培養システム; クラスタリング; 細胞株; 幹細胞; 遺伝子解析; 再生医療; 細胞移植

Human embryonic stem(ES) cells are difficult to be cultivated in vitro, compared with murine ES cells. In contrast, human embryonal carcinoma(EC) cells can easily be cultivated, compared with human ES cells. In this study, we evaluated human EC cell lines to investigate which human cells can be used as feeder cells. We set specification of human feeder cells in terms of maintenance of human ES cells and preservation of pluripotency. We determined human cell types that are capable of being feeder cells and indeed established human feeder cells.

与小鼠胚胎干细胞相比，人胚胎干细胞在体外培养较为困难。相比之下，与人类ES细胞相比，人类胚胎癌细胞(EC)更容易培养。在这项研究中，我们评估了人类EC细胞系，以了解哪些人类细胞可以用作饲养细胞。我们从维持人ES细胞和保持多能性方面设定了人饲养层细胞的规格。我们确定了能够成为饲养细胞的人类细胞类型，实际上已经建立了人类饲养细胞。