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Does prevention of metabolic syndrome lead to improvement of erectile dysfunction or lower urinary tract symptoms?

预防代谢综合征是否可以改善勃起功能障碍或下尿路症状？

基本信息

批准号：
21659370
负责人：
YOKOYAMA Osamu
金额：
$ 2.02万
依托单位：
University of Fukui
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

We investigated whether the prevention of metabolic syndrome led to improvement of erectile dysfunction and lower urinary tract dysfunction. As animal models of metabolic syndrome, we used OLETF(Otsuka-Evans Tokushima Fatty) rats and Dahl salt-sensitive(DSs) rats, and investigated the function of the lower urinary tract.1. The average body weight of male OLETF rats was significantly increased from the age of 24 weeks to 40 weeks, but significantly decreased from the age of 64 weeks. The systolic blood pressure was gradually increased from the age of 24 weeks. Plasma triglyceride, cholesterol, insulin and glucose were significantly higher in OLETF rats than in LETO rats both at the age of 24 and 56 weeks. These characteristics suggest that OLETF rat could be a model in evaluating the relationship between MetS and LUTS.2. The urinary frequency for 24 h was significantly greater in OLETF rats from the age of 24 weeks, and the mean voided volume was significantly greater from the age of 56 … More weeks. This suggests that the male OLETF rats have micturition characteristics of frequent micturition and polyuria from early stage to late stage.3. On cystometry, non-voiding contractions(NVCs) was observed in OLETF rats at the age of 24 weeks, and markedly increased at the age of 56 weeks. The bladder weight, maximum bladder capacity and residual urine were significantly increased at the age of 56 weeks.4. The amount of ATP, PGE2 and NGF released from 20 cm H2O pressure-stretched bladder of OLETF rats was, respectively, significantly increased 3. 87±0. 46, 2. 80±0. 34, 1. 37±0. 22 times as much as those of LETO rats. The amount of 8-OHdG in the bladder of OLETF rats was 4. 71±0. 68 times higher than that of LETO rats.5. The sympathetic nerve activity is augmented with salt loading in Dahl salt-sensitive rats(DSs), but not in Dahl salt-resistant rats(DRs). Six-week-old male DSs and DRs were fed with a normal(0. 3%) or high-(8%) salt diet and 40ml water/day for 12 weeks. Blood pressure was measured through the tail artery under non-anesthetizing state. Urine volume and frequency were recorded all day long in metabolic cage. We calculated inactive period(sleeping) urine volume index(IUi), which was defined as urine volume during inactive period divided by 24-h urine output. Twenty to 30 mmHg increase in systolic and diastolic blood pressure was noted in DSs after 2 week-salt loading. Salt loading increased urine production during active(awake) period of the day both in DSs and DRs. In inactive period, mean voided volume gradually increased in DRs fed with normal or high salt diet, while did not change in DSs fed with high-salt diet. There were significant differences in mean voided volume after 10 week-salt loading between DRs and DSs fed with high-salt diet. In DSs fed with high-salt diet, IUi increased 2 weeks after salt loading, and remained normal range within 4 weeks. Less

我们调查了代谢综合征的预防是否导致勃起功能障碍和下尿路功能障碍的改善。作为代谢综合征的动物模型，我们使用OLETF(Otsuka-Evans Tokushima Fatty)大鼠和Dahl盐敏感(DSS)大鼠，观察下尿路的功能。雄性OLETF大鼠的平均体重从24周龄到40周龄显著增加，但从周龄开始显著下降。从24周龄开始，血压逐渐升高。24周龄和56周龄OLETF大鼠血浆甘油三酯、胆固醇、胰岛素和血糖均显著高于LETO大鼠。这些特点提示OLETF大鼠可作为评价MET与LUTS2关系的模型。OLETF24小时尿频从24周龄开始显著增加，平均排尿量从56…开始显著增加再过几个星期。提示OLETF雄性大鼠从早期到晚期具有尿频、多尿的排尿特点。OLETF大鼠24周龄时可见无排尿收缩(NVC)，56周龄时显著增加。膀胱重量、最大膀胱容量和残余尿量在56周龄时显著增加。OLETF大鼠20cmH2O加压后膀胱释放的三磷酸腺苷、前列腺素E_2和神经生长因子的量分别显著增加3.87±0。46，2.80±0。34，1.37±0。是Leto大鼠的22倍。OLETF大鼠膀胱内8-OHdG含量为4.71±0。比Leto老鼠高68倍。Dahl盐敏感大鼠(DSS)的交感神经活动随盐负荷的增加而增强，而Dahl耐盐大鼠(DRS)的交感神经活动不随盐负荷的增加而增强。6周龄雄性DSS和DRS饲喂正常(0。3%)或高盐饮食(8%)加40ml水/日，共12周。在非麻醉状态下通过尾动脉测量血压。在代谢笼中全天记录尿量和尿频。计算非活动期(睡眠)尿量指数(IUI)，定义为非活动期尿量除以24小时尿量。盐负荷2周后，DSS患者的收缩压和舒张压升高了20~30毫米汞柱。在DSS和DRS中，盐负荷增加了一天中活跃(清醒)时期的尿量。在非活动期，正常饮食和高盐饮食的DSS的平均排尿量逐渐增加，而高盐饮食的DSS的平均排尿量没有变化。高盐饮食组和高盐饮食组大鼠在盐负荷10周后的平均排尿量差异有统计学意义。高盐饮食组大鼠的IUI在盐负荷后2周开始升高，4周内基本维持在正常范围。较少