Development of antibody screening system in mammalian cells using chimeric receptors

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In this study, we aimed to develop a novel method to rapidly and easily select antigen-specific antibody from an antibody library based on growth activity of mammalian cells. To achieve this, fusion proteins composed of an antibody library and a growth-inducing receptor were expressed on mammalian cells, and the resultant cells were cultured in the medium containing a dimeric target antigen, followed by analysis of antigen binding and specificity of the selected cells. As a result, antigen-binding antibody fragments were successfully obtained based on the cell growth.