The study for development of novel in vitro oocyte maturation technique generated from expression profile of micro-RNA during ovulation process

排卵过程中微小RNA表达谱产生的新型体外卵母细胞成熟技术的开发研究

• 批准号: 21688019
• 负责人: SHIMADA Masayuki
• 金额: $ 16.97万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21688019/
• 关键词: 排卵; 顆粒膜細胞; 転写制御; シグナル伝達経路; 受精; 成長因子; 卵; 体外成熟培養; 卵成熟; シグナル伝達; siRNA; 卵巣; 減数分裂; IVM; 遺伝子発現; 卵丘細胞; neureulin 1; ERK1; 2

During ovulation process, ERK1/ 2 pathway increased the expression level of multiple kinds of genes in granulosa cells, which induced luteinization of granulosa cells, cumulus cell expansion and oocyte maturation. However, in in vitro culture system, the level of ERK1/ 2-target genes was lower than those in in vivo due to the low level of neuregulin 1. Neuregulin 1 that enhanced the phosphorylation of ERK1/ 2 was corded by Nrg1 which had two transcription start sites in both terminals. The micro-RNA that might be generated from antisense strand of Nrg1 via Dicer-dependent manner suppressed the transcription of sense strand. To rescue the low level of Nrg1 in in vitro, the addition of neuregulin improved oocyte developmental competence

在排卵过程中，ERK 1/ 2信号通路通过增加颗粒细胞多种基因的表达水平，诱导颗粒细胞黄素化、卵丘细胞扩张和卵母细胞成熟。然而，在体外培养系统中，由于neuregulin 1的低水平，ERK 1/ 2靶基因的水平低于体内。神经调节蛋白1（Neuregulin 1）是一种增强ERK 1/ 2磷酸化的蛋白质，由两端具有两个转录起始位点的Nrg 1编码。Nrg 1反义链可能通过Dicer依赖的方式产生的micro-RNA抑制正义链的转录。为了挽救体外低水平的Nrg 1，添加Neuregulin提高了卵母细胞的发育能力

项目成果

Cumulus cells are an essential mediator of ovulation stimuli from granulosa cells to oocyte (Review article)

卵丘细胞是从颗粒细胞到卵母细胞的排卵刺激的重要介质（评论文章）

• 发表时间: 2010
• 期刊: J Mamm Ova Res (In press)
• 作者: Nazir KHMN. H.;Ichinose H.;Wariishi H.;Sayaka Wakayama;黄川田隆洋;Shimada M
• 通讯作者: Shimada M

IL6: an autocrine regulator of the mouse cumulus cell-oocyte complex expansion process.

IL6：小鼠卵丘细胞-卵母细胞复合体扩张过程的自分泌调节剂。

• 发表时间: 2009
• 期刊: Endocrinology 150
• 作者: Hiromichi Mitamura;Ayumi Doi;Aki Miyagi;Keiichi Uchida;Yoshinori Miyamoto;Toshiharu Kakihara;Yuuki Kawabata;Kotaro Ichikawa;Nobuaki Arai;Zhilin Liu
• 通讯作者: Zhilin Liu

Murine Sperm Expresses Toll-Like Receptor (TLR) Family that Responds to the Pathogens Released from Virus, and Decreases Fertilization Ability by the Stimuli

小鼠精子表达 Toll 样受体 (TLR) 家族，该家族对病毒释放的病原体做出反应，并通过刺激降低受精能力

• 发表时间: 2010
• 作者: Toshihiro Mihara;Keita Fukumoto;T. Okazaki;M. Shimada
• 通讯作者: M. Shimada

凍結精液の作製とその利用法を考える-「きちんと凍結する」,「上手に融かす」,「最適条件で受(授)精させる」-

思考冷冻精液的生产以及如何使用它 - “正确冷冻”、“充分解冻”和“在最佳条件下授精”。

• 发表时间: 2010
• 作者: Takahashi;A.; Kondoh;M.; Uchida;H.; Kakamu;Y.; Hamakubo;T.; Yagi;K.;島田昌之
• 通讯作者: 島田昌之

The innate immune functions of male and female germ cells -The expression of TLR family reduces the risk of infection problem from fetus

男性和女性生殖细胞的先天免疫功能 - TLR家族的表达降低胎儿感染问题的风险

• 发表时间: 2010
• 作者: Nagai K;Tanida M;Niijima A;Tsuruoka N;Kiso Y;Horii Y;Shen J;Okumura N;島田昌之
• 通讯作者: 島田昌之