Physiological and molecular biological studies on acquisition of auditory responsiveness in zebrafish

斑马鱼听觉反应能力获得的生理和分子生物学研究

• 批准号: 22300126
• 负责人: ODA Yoichi
• 金额: $ 10.65万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22300126/
• 关键词: 内耳; 有毛細胞; 耳石; 球形嚢; 卵形嚢; マウスナー細胞; カリウムチャネル; ゼブラフィッシュ; 網様体脊髄路ニューロン; キンギョ; 聴覚; 触覚; 逃避運動; 機械受容; 発火パターン

The developmental origin of inner ear hair cells, role of ear stone size on acoustic sensitivity and molecular mechanisms underlying developmental change in firing properties of hindbrain neurons were studied in vivo in zebrafish embryos and larvae. We demonstrated first that tether cells, a few ciliated cells associated with an ear stone (otolith) in the embryonic inner ear, morphologically and functionally differentiate into first hair cells. Second, the saccule inner ear organ containing a large otolith is necessary to detect sound, whereas the utricle containing a small otolith is not. But, utricle hair cells with experimentally enlarged otoliths acquired the sense of sound, suggesting that otolith size is crucial to sense sound. Third, unique firing properties of Mauthner cells among the hindbrain reticulospinal neurons receiving auditory nerve inputs are acquired during early development and coexpression of Kv beta subunits with low-threshold Kv1 subunits is required for them.

在斑马鱼胚胎和幼虫体内研究了内耳毛细胞的发育起源、耳石大小对声敏感性的影响以及后脑神经元放电特性发育变化的分子机制。我们首先证明了系索细胞，即胚胎内耳中与耳石（耳石）相关的一些纤毛细胞，在形态和功能上分化为第一毛细胞。第二，含有大耳石的囊状内耳器官是探测声音所必需的，而含有小耳石的耳室则不是。但是，实验中耳石增大的耳室毛细胞获得了声音的感觉，这表明耳石的大小对声音的感觉至关重要。第三，在接受听神经输入的后脑网状脊髓神经元中，Mauthner细胞的独特放电特性是在发育早期获得的，Kv β亚基与低阈值Kv1亚基的共表达是它们所必需的。

项目成果

Electrophysiologicalapproaches to study brain functions inzebrafish

研究斑马鱼大脑功能的电生理学方法

• 发表时间: 2012
• 作者: Yuichi Takeuchi;Michio Hori;YoichiOda.;Yoichi Oda;Yoichi Oda;Yoichi Oda
• 通讯作者: Yoichi Oda

Glycinergic transmissions are required for formation and maintenance of synaptic glycine receptor clusters in zebrafish Mauthner cells in vivo

斑马鱼莫特纳细胞体内突触甘氨酸受体簇的形成和维持需要甘氨酸能传递

• 发表时间: 2012
• 作者: Iori Yamanaka;Mariko Miki;Kazuhide Asakawa;Koichi Kawakami;Yoichi Oda;Hiromi Hirata
• 通讯作者: Hiromi Hirata

Neurophysiological basis foracquisition of auditory responsivenessin developing zebrafish

发育中的斑马鱼获得听觉反应能力的神经生理学基础

• 发表时间: 2011
• 作者: Masashi Tanimoto;Maya Inoue;YoichiOda
• 通讯作者: YoichiOda

Large-otolith-mediated mechanotransduction and afferent neural transmission in saccular pathway underlie auditory perception in larval zebrafish

囊状通路中大耳石介导的机械转导和传入神经传递是斑马鱼幼虫听觉感知的基础

• 发表时间: 2012
• 作者: Maya Inoue;Masashi Tanimoto;Yoichi Oda
• 通讯作者: Yoichi Oda

Acquisition of the sense ofhearing during development

在发育过程中获得听觉

• 发表时间: 2012
• 作者: Yuichi Takeuchi;Michio Hori;YoichiOda.;Yoichi Oda;Yoichi Oda;Yoichi Oda;Yoichi Oda;Yoichi Oda
• 通讯作者: Yoichi Oda