Analysis of transcriptional regulation cascade of organic solvent tolerant yeasts and its application

由于化学反应的溶剂通常对催化载体生物具有高毒性，因此有机溶剂耐受性是有效生物生产所必需的。所鉴定的基因的特异性表达将提高实际应用中对特定有机溶剂的耐受性。我们证明了PDR1 R821S突变体对亲水性有机溶剂，DMSO以及疏水性有机溶剂具有耐受性。此外，我们鉴定并区分了编码ABC转运蛋白和细胞壁蛋白的基因，这些基因参与了对疏水或亲水有机溶剂的耐受。

As solvents for the chemical reactions are usually highly toxic to the catalytic carrier organism, organic solvent tolerance is necessary for effective bioproduction. Specific expression of the identified genes would improve the tolerance to particular organic solvents for practical use. We demonstrated the PDR1 R821S mutantis tolerant to hydrophilic organic solvent, DMSO, as well as hydrophobic organic solvents. Furthermore, we identified and discriminated genes encoding ABC transporters and cell wall proteins involved in tolerance to hydrophobic or hydrophilic organic solvents.