Analysis of transcriptional regulation cascade of organic solvent tolerant yeasts and its application

耐有机溶剂酵母转录调控级联分析及其应用

• 批准号: 22360342
• 负责人: UEDA Mitsuyoshi
• 金额: $ 12.48万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22360342/
• 关键词: 有機溶媒耐性; 転写制御因子; ストレス応答; ABC トランスポーター; PDR1 変異; 薬剤耐性; 情報伝達システム; 相互作用; ABCトランスポーター; PDR1変異; 生体触媒; 転写因子; 分子育種; 細胞表層; 相互作用因子

As solvents for the chemical reactions are usually highly toxic to the catalytic carrier organism, organic solvent tolerance is necessary for effective bioproduction. Specific expression of the identified genes would improve the tolerance to particular organic solvents for practical use. We demonstrated the PDR1 R821S mutantis tolerant to hydrophilic organic solvent, DMSO, as well as hydrophobic organic solvents. Furthermore, we identified and discriminated genes encoding ABC transporters and cell wall proteins involved in tolerance to hydrophobic or hydrophilic organic solvents.

由于用于化学反应的溶剂通常对催化载体有机体具有高度毒性，因此有机溶剂耐受性对于有效的生物生产是必要的。已鉴定基因的特异性表达将提高实际应用中对特定有机溶剂的耐受性。我们证明了PDR1R821S突变株对亲水性有机溶剂、DMSO以及疏水性有机溶剂的耐受性。此外，我们还鉴定和区分了编码ABC转运蛋白和细胞壁蛋白的基因，这些基因参与了对疏水性或亲水性有机溶剂的耐受。

项目成果

Functional analysis of Pdr1p in multidrug resistance and organic-solvent tolerance in yeast

Pdr1p在酵母多药耐药和有机溶剂耐受中的功能分析

• 发表时间: 2011
• 作者: K. Oshima;T. Shinagawa;M. Haraguchi;Y. Sekine;N. Nshida et al.
• 通讯作者: N. Nshida et al.

Enhancement of beta-glucosidase activity on the cell-surface of sake yeast by disruption of SED1

通过破坏 SED1 增强清酒酵母细胞表面的 β-葡萄糖苷酶活性

• 发表时间: 2010
• 期刊: J.Biosci.Bioeng.
• 作者: A.Kotaka;et al.
• 通讯作者: et al.

Molecular analysis of XBD in Pdr1p mutants leading to multidrug resistance and organic-solvent tolerance in yeast Saccharomyces cerevisiae

Pdr1p 突变体中 XBD 的分子分析导致酿酒酵母多药耐药性和有机溶剂耐受性

• 发表时间: 2010
• 作者: A. Noujima;T. Mitsudome;T. Mizugaki;K. Jitsukawa;K. Kaneda;松方正彦・豊田真琴・古畑泰介・関根泰・菊地英一;松方正彦・楢島雅俊・今西勇平・関根泰・菊地英一;大草寛之,朴恵珍,高橋剛,堤浩,湯浅英哉,三原久和;N. Nishida et al.
• 通讯作者: N. Nishida et al.

Direct fermentation of newspaper after laccase-treatment using yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase

• DOI: 10.1016/j.renene.2012.01.078
• 发表时间: 2012-08-01
• 期刊: RENEWABLE ENERGY
• 影响因子: 8.7
• 作者: Nakanishi, Akihito;Kuroda, Kouichi;Ueda, Mitsuyoshi
• 通讯作者: Ueda, Mitsuyoshi