Construction of vascular network in regenerative tissue by using angiogenic materials.

利用血管生成材料构建再生组织中的血管网络。

• 批准号: 22390244
• 负责人: KOYAMA Hiroyuki
• 金额: $ 9.07万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390244/
• 关键词: 人工臓器学; 再生医学; 血管新生; 再生組織; 栄養血管; スフェロイド; 膵島移植

Three-dimensional (3-D) regenerative tissue with a certain bulk cannot maintain whole viability in vivo without sufficient blood perfusion. In the present study, we first established a technology to construct neovascular network around small tissue elements (spheroids) by combining with “angiogenic gel”. Angiogenic gel has a unique function to rapidly induce neovascular growth from recipient bed into the inside of the material. Subsequently, we created a new-type angiogenic gel which could also function as a scaffold to support 3-D accumulation of spheroids. A bulky regenerative tissue consisting of accumulated spheroids and the new-type gel was transplanted in vivo to attempt a construction of vascular network covering whole part of the tissue, though viability of spheroids declined in the central part of the tissue. To preserve the viability of all spheroids in the regenerative tissue, we developed anin-gel perfusion system, and a perfusion trial using culture medium showed effectiveness of the system.

具有一定体积的三维（3d）再生组织如果没有足够的血液灌注，就不能在体内保持完整的活力。在本研究中，我们首先建立了一种结合“血管生成凝胶”在小组织元件（球体）周围构建新血管网络的技术。血管生成凝胶具有独特的功能，可以快速诱导新生血管从受体床生长到材料内部。随后，我们创造了一种新型血管生成凝胶，它也可以作为支架来支持球体的三维积累。在体内移植一个由累积的球体和新型凝胶组成的庞大的再生组织，试图构建覆盖整个组织的血管网络，尽管球体在组织中心部分的活力下降。为了保持再生组织中所有球体的活力，我们开发了凝胶灌注系统，并用培养基进行了灌注试验，结果表明了该系统的有效性。

项目成果

A Calcium-Cross-Linked Hydrogel Based on Alginate-Modified Atelocollagen Functions as a Scaffold Material

• DOI: 10.1163/092050611x555678
• 发表时间: 2012-01
• 期刊: Journal of Biomaterials Science, Polymer Edition
• 作者: W. Kamimura;Rieko Hattori;H. Koyama;T. Miyata;T. Takato

Inverse association between the existence of coronary artery disease and progression of abdominal aortic aneurysm

冠状动脉疾病的存在与腹主动脉瘤进展之间呈负相关

• DOI: 10.1016/j.atherosclerosis.2012.02.031
• 发表时间: 2012
• 期刊: Atherosclerosis
• 影响因子: 5.3
• 作者: Nakayama A;Miyata T
• 通讯作者: Miyata T

組織再生用三次元細胞培養システム：オリゴ糖酸化物－アテロコラーゲンゲルの開発

用于组织再生的三维细胞培养系统：低聚糖氧化物-滑胶原凝胶的开发

• 作者: 上村渉;小山博之
• 通讯作者: 小山博之

Enhanced Neovascular Formation in a Novel Hydrogel Matrix Consisting of Citric Acid and Collagen

由柠檬酸和胶原蛋白组成的新型水凝胶基质增强新血管形成

• DOI: 10.3400/avd.oa.11.00017
• 发表时间: 2011
• 期刊: Annals of Vascular Diseases
• 影响因子: 0.8
• 作者: Nagayoshi M;Koyama H;Miyata T
• 通讯作者: Miyata T

酸化オリゴ糖で架橋したゲル材料

氧化寡糖交联的凝胶材料

• 发表时间: 2012