Construction of vascular network in regenerative tissue by using angiogenic materials.

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Three-dimensional (3-D) regenerative tissue with a certain bulk cannot maintain whole viability in vivo without sufficient blood perfusion. In the present study, we first established a technology to construct neovascular network around small tissue elements (spheroids) by combining with “angiogenic gel”. Angiogenic gel has a unique function to rapidly induce neovascular growth from recipient bed into the inside of the material. Subsequently, we created a new-type angiogenic gel which could also function as a scaffold to support 3-D accumulation of spheroids. A bulky regenerative tissue consisting of accumulated spheroids and the new-type gel was transplanted in vivo to attempt a construction of vascular network covering whole part of the tissue, though viability of spheroids declined in the central part of the tissue. To preserve the viability of all spheroids in the regenerative tissue, we developed anin-gel perfusion system, and a perfusion trial using culture medium showed effectiveness of the system.