Highly Assay of Autoantigens in BSE Sera for a Diagnosis

本研究旨在应用四极杆质谱选择反应监测技术（SRM）检测牛海绵状脑病（BSE）的血清生物标志物胶质纤维酸性蛋白（GFAP）。SRM条件由MS/MS片段化的数据确定，并通过Q极MS的数据依赖性MS/MS分析鉴定蛋白质。通过使用蛋白质的烷基化和从血清中去除白蛋白的制备方法高度回收添加剂GFAP。本研究报道的SRM方法具有较高的选择性和灵敏度，可用于已知蛋白质和多肽的筛选。

Our objective is to detect a serum biomarker Glial Fibrillary Acidic Protein (GFAP) of Bovine Spongiform Encephalopathy (BSE) by the Selected Reaction Monitoring (SRM) of quadrupole MS analysis from a bovine serum. SRM conditions were determined from the data of MS/MS fragmentation and identification of the protein by data dependent MS/MS analysis of Q-pole MS. An additive GFAP was highly recovered by the preparation method using alkylation of the proteins and removal of the albumin from the sera. SRM methods reported in this study could identify protein and peptide with high selectivity and sensitivity, therefore this method was useful for screening of known proteins and peptides.