Highly Assay of Autoantigens in BSE Sera for a Diagnosis

高度检测 BSE 血清中的自身抗原以进行诊断

• 批准号: 22580353
• 负责人: YOKOTA Hiroshi
• 金额: $ 2.91万
• 依托单位: Rakuno Gakuen University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580353/
• 关键词: BSE; 生前診断; GFAP; 血清蛋白質; 質量分析; 血清診断; 自己抗体; 抗原検出

Our objective is to detect a serum biomarker Glial Fibrillary Acidic Protein (GFAP) of Bovine Spongiform Encephalopathy (BSE) by the Selected Reaction Monitoring (SRM) of quadrupole MS analysis from a bovine serum. SRM conditions were determined from the data of MS/MS fragmentation and identification of the protein by data dependent MS/MS analysis of Q-pole MS. An additive GFAP was highly recovered by the preparation method using alkylation of the proteins and removal of the albumin from the sera. SRM methods reported in this study could identify protein and peptide with high selectivity and sensitivity, therefore this method was useful for screening of known proteins and peptides.

我们的目的是通过四极杆MS分析的选择性反应监测(SRM)从牛血清中检测牛海绵状脑病(BSE)的血清生物标记物胶质纤维酸性蛋白(GFAP)。根据MS/MS裂解数据确定SRM条件，并通过Q-POLE MS数据依赖MS/MS分析鉴定蛋白质，通过蛋白质烷基化和从血清中去除白蛋白的制备方法获得了高回收率的添加剂GFAP。本研究报道的SRM方法可以高选择性和高灵敏度地鉴定蛋白质和多肽，因此该方法对于筛选已知的蛋白质和多肽是有用的。

项目成果

メタボローム解析によるビスフェノールAの妊娠期暴露影響の新規探索

通过代谢组分析新探索妊娠期间双酚A暴露的影响

• 发表时间: 2010
• 作者: 岩野英知;西川美宇;森田安奈;柳沢梨沙;封馬澄人;井上博紀;横田博
• 通讯作者: 横田博

Relationships between Carapace Sizes and Plasma Major and Trace Element Status in Captive Hawksbill Sea Turtles (<i>Eretmochelys imbricata</i>)

圈养玳瑁海龟 (<i>Eretmochelys imbricata</i>) 的甲壳大小与血浆主要元素和微量元素状态之间的关系

• DOI: 10.1292/jvms.12-0099
• 发表时间: 2012
• 期刊: Journal of Veterinary Medical Science
• 影响因子: 1.2
• 作者: Kazuyuki SUZUKI*;Jun NODA;Makio YANAGISAWA;Isao KAWAZU;Kouichiro SERA;Daisuke FUKUI;Mitsuhiko ASAKAWA and Hiroshi YOKOTA.
• 通讯作者: Mitsuhiko ASAKAWA and Hiroshi YOKOTA.

A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle

• DOI: 10.4142/jvs.2011.12.2.191
• 发表时间: 2011-06-01
• 期刊: JOURNAL OF VETERINARY SCIENCE
• 影响因子: 1.8
• 作者: Higuchi, Hidetoshi;Iwano, Hidetomo;Nagahata, Hajime
• 通讯作者: Nagahata, Hajime

Biochemical and Immunohistochemical Characterization of the Amyloid in Canine Amyloid-Producing Odontogenic Tumor

• DOI: 10.1177/0300985810375047
• 发表时间: 2010-09-01
• 期刊: VETERINARY PATHOLOGY
• 影响因子: 2.4
• 作者: Hirayama, K.;Miyasho, T.;Taniyama, H.
• 通讯作者: Taniyama, H.

High Mobility Group Box 1 (HMGB1) Protein is Present in the Cerebrospinal Fluid of Dogs with Encephalitis

• DOI: 10.1292/jvms.10-0444
• 发表时间: 2011-07-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Miyasho, Taku;Nakamura, Kozo;Yokota, Hiroshi
• 通讯作者: Yokota, Hiroshi