M0LECULAR MECHANISIM AND BIOMARKER OF AORTIC DISSECTION

主动脉夹层的分子机制和生物标志物

• 批准号: 22591535
• 负责人: SATO Akira
• 金额: $ 2.91万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591535/
• 关键词: B型大動脈解離; テネイシンCノックアウトマウス; バイオマーカー; トランクスクリプトーム解析

We evaluated 42 patients with type B acute aortic dissection (AD), andserum TN-C levels significantly increased at 7 days and then gradually decreased to normal range at 28 days. Serum TN-C levels at 7 days correlated positively with peak C-reactive protein levels, FDP levels, D-dimer levels, and maximum aortic diameter at the dissection level on admission. Serum TN-C levels of 3 dead patients on admission were significantly higher than those of surviving patients. Serum TN-C levels were significantly elevated during acute stage and might be a novel biomarker to predict patient prognosis of type B acute AD.In mouse model, TN-C was highly expressed in the medial layer of the dissected aortic wall, andwas concordant with the area of the vascular smooth muscle cells (VSMCs) stained by α-SMA. In the TN-C reporter mouse knock-in with LacZ, the medial layer of the dissected aortic wall was stained by the β-gal, and furthermore αSMA-positive cells were also positive in β-gal. We found that the producing cells of TN-C were VSMCs in the dissected aortic wall.

我们对42例B型急性主动脉夹层(AD)患者进行了评估，发现血清Tn-C在第7天显著升高，在第28天逐渐下降至正常范围。入院时第7天的血清TN-C水平与峰值C反应蛋白水平、FDP水平、D-二聚体水平和夹层水平的最大主动脉直径呈正相关。3例死亡患者入院时血清Tn-C水平明显高于存活患者。血清Tn-C水平在急性B型AD急性期显著升高，可能成为预测B型急性AD患者预后的新的生物标志物。在小鼠模型中，Tn-C在夹层的主动脉壁中层高度表达，并与α-SMA染色的血管平滑肌细胞面积一致。在用LacZ敲击TN-C报告基因的小鼠中，β-GAL染色显示夹层的主动脉壁中层，α-GAL阳性细胞也呈β-GAL阳性。我们发现在夹层的主动脉壁中产生TN-C的细胞是VSMCs。

项目成果

テネイシンCは大動脈解離を予防する分子的ショックアブソーバーである

Tenascin-C 是一种分子减震器，可预防主动脉夹层

• 发表时间: 2011
• 作者: 片山雄三;横山詩子;根元寛子;笠間啓一郎;鈴木伸一;磯松尚幸;内田敬二;井元清隆;石川義弘;益田宗孝;木村泰三,吉村耕一,青木浩樹,今中恭子,吉田利通,青沼和隆,廣江道昭,今泉勉,松崎益徳
• 通讯作者: 木村泰三,吉村耕一,青木浩樹,今中恭子,吉田利通,青沼和隆,廣江道昭,今泉勉,松崎益徳

大動脈瘤分子病態における細胞外マトリックステネイシンCの役割の解明

阐明细胞外基质腱蛋白-C 在主动脉瘤分子病理学中的作用

• 发表时间: 2010
• 作者: 鈴木伸一、益田宗孝、磯松尚幸、笠間啓一郎、片山雄三、根元寛子、井元清隆、内田敬二、南智行、堺温哉;松本直通;木村泰三,佐藤 明,青沼和隆
• 通讯作者: 木村泰三,佐藤 明,青沼和隆

Tenascin-C is expressed in abdominal aortic aneurysm tissue with an active degradation process

• DOI: 10.1111/j.1440-1827.2011.02699.x
• 发表时间: 2011-10-01
• 期刊: PATHOLOGY INTERNATIONAL
• 影响因子: 2.2
• 作者: Kimura, Taizo;Yoshimura, Koichi;Matsuzaki, Masunori
• 通讯作者: Matsuzaki, Masunori