Elucidation of molecular basis of specificity in biosynthetic regulation and action mechanisms of antimicrobial peptides from lactic acid bacteria

阐明乳酸菌抗菌肽生物合成调节特异性的分子基础和作用机制

• 批准号: 22780069
• 负责人: ZENDO Takeshi
• 金额: $ 2.58万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780069/
• 关键词: 微生物機能; 乳酸菌; 抗菌ペプチド; バクテリオシン

Structures of antimicrobial peptides, bacteriocins, from lactic acid bacteria were analyzed, and some novel bacteriocins such as enterocin W, leucocyclicin Q and weissellicins YM were identified. Biosynthetic genes and their functions of some bacteriocins such as lacticin Q, enterocins NKR-5-3 and weissellicins YM were analyzed, and their biosynthetic regulation systems were partly unraveled. Action mechanisms of some bacteriocins were studied, and some factors for their specific antimicrobial actions were revealed.

对乳酸菌抗菌肽细菌素的结构进行了分析，鉴定出一些新的细菌素，如肠霉素W、白细胞环素Q和魏丝霉素YM。分析了乳素Q、肠素NKR-5-3、微乳素YM等细菌素的生物合成基因及其功能，部分揭示了它们的生物合成调控系统。对几种细菌素的作用机制进行了研究，揭示了影响其特异性抑菌作用的因素。

项目成果

Analysis of gene cluster involved in production of the circular bacteriocin leucocylicin Q by Leuconostoc mesenteroides TK41401

肠膜明串珠菌TK41401产生环状细菌素白环素Q的基因簇分析

• 发表时间: 2012
• 作者: Mu F.;Masuda Y.;Ono H.;Kitagawa H.;Ito H.;Zendo T.;Nakayama J.;Sonomoto K.
• 通讯作者: Sonomoto K.

Biosynthesis mechanism of lacticin Q from Lactococcus lactis QU 5

乳酸乳球菌QU 5乳酸素Q的生物合成机制

• 发表时间: 2011
• 作者: Zendo T.;Iwatani S.;Yoneyama F.;Nakayama J.;Sonomoto K.
• 通讯作者: Sonomoto K.

Isolation and Characterization of Enterocin W, a Novel Two-Peptide Lantibiotic Produced by Enterococcus faecalis NKR-4-1

• DOI: 10.1128/aem.06497-11
• 发表时间: 2012-02-01
• 期刊: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
• 影响因子: 4.4
• 作者: Sawa, Naruhiko;Wilaipun, Pongtep;Sonomoto, Kenji
• 通讯作者: Sonomoto, Kenji

Weissellicins YMの環境応答型生産制御機構の解明

阐明 Weissellins YM 的环境响应生产控制机制

• 发表时间: 2012
• 作者: 益田時光;善藤威史;Perez R. H.;園元謙二
• 通讯作者: 園元謙二

Identification and Characterization of Leucocyclicin Q, a Novel Cyclic Bacteriocin Produced by Leuconostoc mesenteroides TK41401

• DOI: 10.1128/aem.06348-11
• 发表时间: 2011-11-01
• 期刊: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
• 影响因子: 4.4
• 作者: Masuda, Yoshimitsu;Ono, Hiroshi;Sonomoto, Kenji
• 通讯作者: Sonomoto, Kenji