Molecular mechanism of neurite outgrowth regulated by PLCdelta3

磷脂酶Cδ3（PLCδ3）是一种在神经组织中高表达的蛋白质，它水解磷脂酰肌醇4，5-二磷酸（PI（4，5）P_2），产生两个第二信使，即磷脂酰肌醇（PI）和磷脂酰肌醇（PI）。e. IP_3和DAG。在E14胚胎的大脑皮层中通过子宫内电穿孔敲低PLCδ3，导致发育中的大脑皮层板中皮层神经元的迁移抑制。此外，我们证明，当PLCδ3在分化过程中被瞬时敲低时，大脑神经元、小脑颗粒细胞或Neuro 2a细胞中的神经元生长受到抑制。

Phospholipase Cδ3(PLCδ3), which is highly expressed in neuronal tissues, hydrolyzes phosphatidylinositol 4, 5-bisphosphate(PI(4, 5) P_2) and generates two second messengers, i. e. IP_3 and DAG. PLCδ3 knockdown in cerebral cortex of E14 embryo by in utero electroporation caused the migratory inhibition of cortical neurons in developing cerebral cortical plate. Furthermore, we demonstrated that neuronal outgrowth was inhibited in cerebral neurons, cerebellar granule cells, or Neuro2a cells when PLCδ3 was transiently knocked down during differentiation.