Interaction Human hepato-cell between Hepatocellular carcinoma specific HBV mutation

我们之前报道hbx94、HBx131和HBx130氨基酸突变与乙型肝炎病毒感染的HCC患者相关。我们构建了表达3hbx转基因野生小鼠（野生TG小鼠（2株）、WM TG小鼠（2株）和MM TG小鼠（3株））。野生TG小鼠为野生HBx表达转基因小鼠。WM TG小鼠HBx， HBx131和HBx130氨基酸突变表达转基因小鼠。MM TG小鼠HBx， hbx94、HBx131和HBx130氨基酸突变表达转基因小鼠。在野生TG小鼠中，肝细胞癌变依赖于HBx的表达量。与野生TG小鼠和WM TG小鼠相比，MM TG小鼠HBX表达量相对较小，但MM TG小鼠发生HCC。基因1和细胞周期蛋白B1 mRNA在表达3hbx的转基因小鼠中的表达高于B6j小鼠。但3种HBx转基因小鼠在这些表达上没有差异。

We previously reported that HBx 94, HBx131 and HBx130 amino acid mutations associate with HCC patients infected with hepatitis B virus. We made wild 3 HBx expressing transgenic mouse(wild TG mouse(2 strain), WM TG mouse(2 strain) and MM TG mouse(3 strain)). Wild TG mouse was wild HBx expressing transgenic mouse. WM TG mouse was HBx with HBx131 and HBx130 amino acid mutations expressing transgenic mouse. MM TG mouse was HBx with HBx 94, HBx131 and HBx130 amino acid mutations expressing transgenic mouse. In wild TG mice, hepatocellular carcinogenesis is dependent on quantity of HBx expression. MM TG mice are relatively smaller expression of HBX than wild TG mouse and WM TG mouse, but HCC developed in MM TG mouse. Gene1(for so it will be convenient to speak of it) and cyclin B1 mRNA were expressed more in 3 HBx expressing transgenic mouse than in B6j mouse. But there was not difference among 3 HBx expressing transgenic mice on these expression.