The relationship between actin bundling by dynamin/cortactin complex and stretch-activated cation channel on actin cytoskeleton

研究了拉伸激活阳离子通道对肌动蛋白动力学的影响。在细胞迁移过程中，皱褶膜中的钙离子浓度升高。癌细胞中通道的敲除导致通过通道的钙流入和血清刺激的皱褶形成的显著减少，这是细胞迁移所必需的。这些结果表明，癌细胞中拉伸激活的阳离子通道可能通过调节肌动蛋白动力学参与细胞迁移。此外，我们确定了动力蛋白/接触蛋白复合物在肌动蛋白动力学调节中的作用。动力蛋白/接触蛋白复合物捆绑了几个肌动蛋白丝，这种捆绑稳定了肌动蛋白丝。动力蛋白/接触蛋白复合物的肌动蛋白捆绑是生长锥丝足的形成和细胞迁移所必需的。这些发现可能对神经元再生和癌细胞迁移的研究具有重要意义。

The function of stretch-activated cation channel on actin dynamics was investigated. Calcium ion concentration in the ruffle membrane was increased during cell migration. Knockdown of the channel in cancer cell line resulted in marked reduction ofboth calcium influx via the channel and serum-stimulated ruffle formation, which were required for cell migration. These results suggest that stretch-activated cation channel in cancer cell is involved in cell migration possibly by regulating actin dynamics. Furthermore, we identified the function of dynamin/cortactin complex in the regulation of actin dynamics. The dynamin/cortactin complexes bundled several actin filaments and the bundling stabilized actin filaments. The actin bundling by dynamin/cortactin complex was necessary for formation of growth cone filopodia and cell migration. These findings might be crucial for the study for neuronal regeneration and cancer cell migration.