Development of High Performance Gene Transfection Methods Using Microplasma Integrated Devices

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We have tried gene transfection experiments using an integrated device composed of microplasma jets. After the irradiation of plasma jet onto COS cells mixed with GFP genes and cultivation of those cells for 24 hours, the GFP genes have been successfully transfected into a certain amount of cells. Through a series of experiments with several kinds of plasma sources, it is concluded that the effects of transported electric charges from plasma produced particles play a key role in the transfection mechanisms. Upon this understanding, we have developed a new electro-spray method in which a higher efficiency of transfection is achieved with suppressing the damages on living cells.