A novel strategy for the generation of anti-glycan antibodies and its application

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Anti-carbohydrate monoclonal antibodies(mAbs) are very useful in the functional analysis of complex carbohydrates in vivo. However, such mAbs are difficult to generate, largely because a wide variety of complex carbohydrates is intrinsically expressed in mice and rats and because the antigenicities of glycans are generally poor. In this study, we developed an efficient method for generating anti-carbohydrate mAbs using glycan-synthesizing enzyme-knockout mice in which the glycan structures formed by the missing enzymes should be highly antigenic. As an application of this method, we generated anti-sulfated glycan mAbs using sulfotransferase-deficient mice and found that sulfated N-and sulfated O-glycans function cooperatively in lymphocyte homing and immune surveillance using those mAbs.