Establishment of an interferon transfer factor prevention cell strainuseful on virus hunting

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The goal of our project is to establish interferon (IFN) signaling-deficient cell lines that are highly susceptible to viral infection, proliferation and survival. In particular, we target those viruses which are proven difficult to isolate by established protocols. We transformed 293T cells with plasmids that have negative effects on various IFN-signal transduction molecules, and subsequently confirmed by monitoring IFN-stimulated genes. Our final goal is to establish iPS cells deficient in IFN signaling. As iPS cells are pluripotent, we hypothesized that once established, we can manipulate these iPS cells to differentiate into different tissue types. This will become a very powerful tool for the isolation of tissue-specific viruses. Currently, we focus our effort in generating iPS cells deficient in IFN signaling. We expect these cells will become available soon and hope to translate it for clinical use in the near future.