Trichloroethylene-induced hypersensitivity may be caused by its active metabolite-protein adducts

三氯乙烯引起的过敏可能是由其活性代谢物-蛋白质加合物引起的

• 批准号: 22659123
• 负责人: NASU Tamie
• 金额: $ 1.96万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659123/
• 关键词: インターフェロンγ; 肝臓; γGTP; 好酸球; CYP2E1; トリクロロエチレン; トリクロロエタノール; トリクロロ酢酸; 皮膚; HSP70; GRP78; Th1; モルモット

We investigated skin and liver damages associated with trichloroethylene-, trichloroethanol-and trichloroacetic acid-induved hypersensitive skin reactions in the hartley guinea pig maximization test. The sensitization rates of trichloroethylene-induced dermal allergy were 70% in male guinea pigs, and 90% in female ones. Those of trichloroethanol were 20% in male guinea and 50% in female ones, while only a minor sensitization was seen after trichloroacetic acid treatment. In relation to the sensitization, eosinocytes were seen in the skin, and infiltration of lymphocytes was seen around focal necrosis in the liver. These changes were seen most prominent in trichloroethylene-exposed animals, followed by trichloroethanol. Trichloroethylene exposure also increased plasmaγGTP and interferonγlevels, while trichloroethanol and trichloroacetic acid did not.

我们研究了在hartley豚鼠最大剂量试验中三氯乙烯、三氯乙醇和三氯乙酸引起的过敏性皮肤反应对皮肤和肝脏的损害。三氯乙烯致敏豚鼠的致敏率，雄性为70%，雌性为90%。三氯乙醇对雄性豚鼠的致敏率为20%，对雌性豚鼠的致敏率为50%，而三氯乙酸处理后仅观察到轻微的致敏作用。与致敏相关，在皮肤中观察到嗜酸性细胞，在肝脏局灶性坏死周围观察到淋巴细胞浸润。这些变化在三氯乙烯暴露的动物中最为突出，其次是三氯乙醇。三氯乙烯暴露还增加了血浆γGTP和γ干扰素水平，而三氯乙醇和三氯乙酸则没有。

项目成果

DIFFERENTIAL RESPONSE TOTRICHLOROETHYLENE-INDUCEDHEPATOSTEATOSIS IN WILD-TYPE AND PPARA-HUMANIZED MICE

野生型和 PPARA 人源化小鼠对三氯乙烯诱导的肝脂肪变性的不同反应

• 发表时间: 2010
• 作者: Ramdhan DH;Kamijima M;Wang D;Ito Y;Yanagiba Y;Hayashi Y;Naito H;Gonzalez FJ;Nakajima T
• 通讯作者: Nakajima T

モルモットを用いたトリクロロエチレンによる皮膚・肝障害のメカニズム解析

三氯乙烯对豚鼠皮肤和肝脏损伤的机制分析

• 发表时间: 2012
• 作者: 内藤久雄;日比野優衣;斉藤優;王棟;趙娜;宋向栄;王海蘭;上島通浩;那須民江
• 通讯作者: 那須民江

トリクロロエチレン誘発性肝障害におけるPPARα活性の種差による影響について

三氯乙烯所致肝损伤中 PPARα 活性的物种差异

• 发表时间: 2010
• 作者: Ramdhan DH,上島通浩,王棟,伊藤由起,柳場由絵,林由美,内藤久雄;Gonzalez FJ,那須民江
• 通讯作者: Gonzalez FJ,那須民江