New strategy for seasonal allergic rhinitis by exosomes

外泌体治疗季节性过敏性鼻炎的新策略

• 批准号: 22659306
• 负责人: FUJIEDA Shigeharu
• 金额: $ 2.01万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659306/
• 关键词: エクソゾーム; スギ花粉症; MHC class II; CD9; CD86; 細胞間連絡物質; MHC classII alpha鎖; CryJ1; ペプチド; 遺伝情報; 蔗糖密度勾配; 樹状細胞

Exosomes are complex structures composed of a lipid bilayer that contains transmembrane proteins and encloses soluble hydrophilic components derived from the cytosol of the donor cells. They also contain the information of antigens with MHC class I & II. These molecules would be taken by neighboring cells and stimulate the receptors in the surface of cells. In this study we examined whether isolated exosomes from good responder of sublingual immunotherapy for allergic rhinitis(Japanese cedar pollen) could have an effect on immune systems. We got 15 to 80μg exosomes from the human sera. Western blots demonstrated that exosomes had MHC class IIαchain, CD9 and CD86 molecules. No band was found by anti-Cry J1(Japanese cedar pollen antigen) antibody in western blots. This study showed that we could isolate exosomes from human sera. However, we need more improvement methods to get high volume of exosomes and sensitivity to detect them for the advanced study.

外泌体是由脂质双层组成的复杂结构，所述脂质双层含有跨膜蛋白并包封来源于供体细胞的胞质溶胶的可溶性亲水组分。它们还包含具有MHC I类和II类的抗原的信息。这些分子会被邻近的细胞吸收，并刺激细胞表面的受体。在这项研究中，我们研究了从过敏性鼻炎舌下免疫疗法（日本雪松花粉）的良好应答者中分离的外泌体是否会对免疫系统产生影响。我们从人血清中获得15至80μg外泌体。Western blot结果显示，exosomes中含有MHC Ⅱ类α链、CD9和CD86分子。用抗Cry J1（日本雪松花粉抗原）抗体进行Western印迹，未发现条带。这项研究表明，我们可以从人血清中分离外泌体。然而，我们需要更多的改进方法，以获得高容量的外泌体和灵敏度来检测它们的进一步研究。

项目成果

New clinical marker for allergic rhinitis

过敏性鼻炎的新临床标志物

• 发表时间: 2011
• 作者: Takahashi M;Kimura Y;Sawabe M;Kitamura K;日高隆雄;Fujieda S
• 通讯作者: Fujieda S

アレルギー性鼻炎

过敏性鼻炎

• 发表时间: 2019
• 作者: 音成 恵梨子;木谷 芳晴;宮崎 和美;松原 彩;鳥居 紘子;山下 勝;高木 明;鈴木元彦
• 通讯作者: 鈴木元彦

B type CpG-DNA suppresses poly (I:C)-induced BLyS expression and production in human tonsillar fibroblasts

B 型 CpG-DNA 抑制聚 (I:C) 诱导的人扁桃体成纤维细胞中 BLyS 的表达和产生

• DOI: 10.1016/j.clim.2011.09.012
• 发表时间: 2011
• 期刊: Clin Immunol
• 影响因子: 8.6
• 作者: Yamada T;et al
• 通讯作者: et al

Prevalence of inhaled antigen sensitization and nasal eosinophils in Japanese children under two years old

• DOI: 10.1016/j.ijporl.2011.10.024
• 发表时间: 2012-02-01
• 期刊: INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY
• 影响因子: 1.5
• 作者: Osawa, Yoko;Suzuki, Dai;Fujieda, Shigeharu
• 通讯作者: Fujieda, Shigeharu

Association between genetic variant of interleukin-33 and seasonal allegic rhinitis in the Japanese population

日本人群中白细胞介素 33 的遗传变异与季节性过敏性鼻炎之间的关联

• 发表时间: 2011
• 作者: Fujieda S;et al.
• 通讯作者: et al.