New strategy for seasonal allergic rhinitis by exosomes

外泌体是由脂质双分子层组成的复杂结构，含有跨膜蛋白，并包裹来自供体细胞细胞质的可溶性亲水性成分。它们还含有MHC I类和II类抗原的信息。这些分子会被邻近的细胞吸收，并刺激细胞表面的受体。在这项研究中，我们研究了从舌下免疫治疗过敏性鼻炎（杉木花粉）中分离的外泌体是否对免疫系统有影响。我们从人类血清中提取了15到80个外泌体。Western blot结果显示外泌体具有MHCⅱ类α链、CD9和CD86分子。western blot结果显示抗J1（杉木花粉抗原）抗体未发现条带。本研究表明，我们可以从人血清中分离出外泌体。然而，我们需要更多的改进方法来获得大量的外泌体和检测它们的灵敏度，以便深入研究。

Exosomes are complex structures composed of a lipid bilayer that contains transmembrane proteins and encloses soluble hydrophilic components derived from the cytosol of the donor cells. They also contain the information of antigens with MHC class I & II. These molecules would be taken by neighboring cells and stimulate the receptors in the surface of cells. In this study we examined whether isolated exosomes from good responder of sublingual immunotherapy for allergic rhinitis(Japanese cedar pollen) could have an effect on immune systems. We got 15 to 80μg exosomes from the human sera. Western blots demonstrated that exosomes had MHC class IIαchain, CD9 and CD86 molecules. No band was found by anti-Cry J1(Japanese cedar pollen antigen) antibody in western blots. This study showed that we could isolate exosomes from human sera. However, we need more improvement methods to get high volume of exosomes and sensitivity to detect them for the advanced study.