Development of microdevices for engineering vascularized tissues

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The purpose of this study is to produce a three-dimensional tissue with hierarchic structures and vascular networks by using the uniform diameter cell beads fabricated using microfulidic devices. In order to form a three-dimensional tissue with a hierarchical structure consisting of multiple cell types, we investigated the distributions of the bead size and the cell types. Furthermore, we have examined the method to introduce vascular networks within the three-dimensional tissues.