Exploration of disease-related proteins from spontaneous model of melanoma by proteomics analysis

通过蛋白质组学分析探索黑色素瘤自发模型中的疾病相关蛋白

• 批准号: 22700457
• 负责人: KAWAMOTO Yoshiyuki
• 金额: $ 2.5万
• 依托单位: Chubu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700457/
• 关键词: 疾患モデル; プロテオミクス解析; メラノーマ; バイオマーカー; RET; 転移; 血清プロテオーム; 悪性黒色腫

RET-transgenic (RET-Tg) mice are useful for identifying melanoma-related biomarkers which can predict tumor progression or metastasis. In this study, we found some serum proteins which may relate to melanoma progression or metastasis by proteomic study. In addition, invasive activity of Mel-ret cells, which were established from RET-tg mice, treated with TNF-alpha were enhanced, and some up- or down-regulated genes were identified by DNA array analysis. 2-Dimensional Fluorescence Difference Gel Electrophoresis (2D-DIGE) analysis revealed differential expression of some proteins between B16 melanoma cell line and the highly metastatic cell line B16-F10. In conclusion, we identified some potential candidate for melanoma-related biomarkers.

RET转基因(RET-TG)小鼠可用于识别可预测肿瘤进展或转移的黑色素瘤相关生物标志物。在本研究中，我们通过蛋白质组学研究发现了一些可能与黑色素瘤进展或转移相关的血清蛋白。此外，从RET-TG小鼠建立的Mel-ret细胞经肿瘤坏死因子-α处理后，其侵袭活性增强，并通过DNA阵列分析鉴定了一些上调或下调的基因。二维荧光差示凝胶电泳法(2D-DGE)分析表明，B16黑色素瘤细胞系与高转移细胞系B16-F10的某些蛋白质表达存在差异。总之，我们确定了一些黑色素瘤相关生物标志物的潜在候选者。

项目成果

RAS/RAF/MEK/ERK and PI3K/PTEN/AKT Signaling in Malignant Melanoma Progression and Therapy.

• DOI: 10.1155/2012/354191
• 发表时间: 2012
• 期刊: Dermatology research and practice
• 影响因子: 1.5
• 作者: Yajima I;Kumasaka MY;Thang ND;Goto Y;Takeda K;Yamanoshita O;Iida M;Ohgami N;Tamura H;Kawamoto Y;Kato M
• 通讯作者: Kato M

Protective role of Gipie, a Girdin family protein, in endoplasmic reticulum stress responses in endothelial cells.

• DOI: 10.1091/mbc.e10-08-0724
• 发表时间: 2011-03-15
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: Matsushita E;Asai N;Enomoto A;Kawamoto Y;Kato T;Mii S;Maeda K;Shibata R;Hattori S;Hagikura M;Takahashi K;Sokabe M;Murakumo Y;Murohara T;Takahashi M
• 通讯作者: Takahashi M

メラノーマ自然発症RETトランスジェニックマウスを用いた腫瘍関連バイオマーカーの探索

使用患有自发性黑色素瘤的 RET 转基因小鼠寻找肿瘤相关生物标志物

• 发表时间: 2011
• 作者: 東岸任弘;中村昇太;朴辰幸;片貝祐子;保富康宏;立花太郎;堀井俊宏;川本善之
• 通讯作者: 川本善之

Control of genetically prescribed protein tyrosine kinase activities by environment-linked redox reactions.

• DOI: 10.4061/2011/896567
• 发表时间: 2011
• 期刊: Enzyme research
• 作者: Nakashima I;Kawamoto Y;Takeda K;Kato M
• 通讯作者: Kato M

A Redox-Linked Novel Pathway for Arsenic-Mediated RET Tyrosine Kinase Activation

• DOI: 10.1002/jcb.22550
• 发表时间: 2010-05-15
• 期刊: JOURNAL OF CELLULAR BIOCHEMISTRY
• 影响因子: 4
• 作者: Kato, Masashi;Takeda, Kozue;Kawamoto, Yoshiyuki
• 通讯作者: Kawamoto, Yoshiyuki