Exploration of disease-related proteins from spontaneous model of melanoma by proteomics analysis

RET转基因(RET-TG)小鼠可用于识别可预测肿瘤进展或转移的黑色素瘤相关生物标志物。在本研究中，我们通过蛋白质组学研究发现了一些可能与黑色素瘤进展或转移相关的血清蛋白。此外，从RET-TG小鼠建立的Mel-ret细胞经肿瘤坏死因子-α处理后，其侵袭活性增强，并通过DNA阵列分析鉴定了一些上调或下调的基因。二维荧光差示凝胶电泳法(2D-DGE)分析表明，B16黑色素瘤细胞系与高转移细胞系B16-F10的某些蛋白质表达存在差异。总之，我们确定了一些黑色素瘤相关生物标志物的潜在候选者。

RET-transgenic (RET-Tg) mice are useful for identifying melanoma-related biomarkers which can predict tumor progression or metastasis. In this study, we found some serum proteins which may relate to melanoma progression or metastasis by proteomic study. In addition, invasive activity of Mel-ret cells, which were established from RET-tg mice, treated with TNF-alpha were enhanced, and some up- or down-regulated genes were identified by DNA array analysis. 2-Dimensional Fluorescence Difference Gel Electrophoresis (2D-DIGE) analysis revealed differential expression of some proteins between B16 melanoma cell line and the highly metastatic cell line B16-F10. In conclusion, we identified some potential candidate for melanoma-related biomarkers.