Optimization of the bone regeneration system using small molecules and examination of its molecular mechanisms

本研究旨在了解成骨小分子调控再生骨形态和数量的骨再生疗法的基础知识。成骨小分子成骨实验的优化结果表明，几个小分子能有效地诱导成骨细胞分化。机制研究提出，它们的作用模式是细胞命运指定为成骨细胞谱系，以及随后的成骨细胞成熟。将这些分子局部注射到骨缺损模型中，可以在动物体内实现骨再生。因此，这些发现将导致基于小分子的骨再生疗法的发展。

This study is aimed to obtain basic knowledge on the bone regenerative therapy in which the shape and amount of regenerated bones are controlled by osteogenic small molecules. Optimization of osteogenic small molecule-based bone formation revealed that several small molecules efficiently induced osteoblast differentiation. Mechanistic studies proposed that their modes of action were the cell fate specification into an osteoblast lineage and the subsequent osteoblast maturation. Local administration of the molecules into bone defect models achieved bone regeneration in animals. Thus, these findings will lead to the development of small molecule-based bone regenerative therapies.