Development of technology for efficient homologous recombination by the viral vector and zincfinger nuclease

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Recent great progress of development of genome editing technology such as ZFN, TALEN, Cas9-CRISPR enabled us to generate gene targeting mice without labor intensive work.These genome editing systems can cut the specific sequence on the genome followed by recruitment of DNA repair machinery to the digested site and enhance the homologous recombination. On the other hand, simultaneous expression of several components are necessary for genome editing by these systems and this is the drawback of these systems for efficient genome editing in primary cells. In this study, we tried to generate single lentiviral vector harboring several components of gene editing system for efficient gene edition.