Molecular mechanism for arrested fork stabilization by fork recognition complex

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Analyses for arrested replication fork recognition by fork stabilizing factor are important for elucidation of molecular mechanism of replication restart reaction. Escherichia coli PriA protein, bacterial fork stabilizing factor which can bind to arrested replication forks, can be used as an indicator for detection of arrested forks in living cells. PriA binds to novel replication origin and replication restart, which is activated by origin-independent system, depends on formation of RNA-DNA hybrid. RNA-dependent initiation system for replication may reflect primitive replication mechanism and these findings shed light on evolution of eukaryotic replication system.