Molecular mechanism for arrested fork stabilization by fork recognition complex

通过叉识别复合物稳定叉的分子机制

• 批准号: 23570183
• 负责人: TANAKA Taku
• 金额: $ 3.41万
• 依托单位: Tokyo Metropolitan Institute of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570183/
• 关键词: DNA複製; 複製起点; 複製再開始; マイクロアレイ; PriA; RecA; 複製フォーク; フォーク安定化因子; DNA結合; 修復・組換え; 分子間相互作用

Analyses for arrested replication fork recognition by fork stabilizing factor are important for elucidation of molecular mechanism of replication restart reaction. Escherichia coli PriA protein, bacterial fork stabilizing factor which can bind to arrested replication forks, can be used as an indicator for detection of arrested forks in living cells. PriA binds to novel replication origin and replication restart, which is activated by origin-independent system, depends on formation of RNA-DNA hybrid. RNA-dependent initiation system for replication may reflect primitive replication mechanism and these findings shed light on evolution of eukaryotic replication system.

叉稳定因子识别被阻滞的复制叉对阐明复制重启反应的分子机制具有重要意义。大肠埃希菌PriA蛋白是一种可以结合到阻滞复制叉上的细菌叉稳定因子，可以作为检测活细胞中阻滞复制叉的指示剂。PriA结合到新的复制起始点上，并通过与起始点无关的系统激活复制重新开始，这取决于RNA-DNA杂交的形成。依赖rna的复制起始系统可能反映了原始的复制机制，这些发现为真核生物复制系统的进化提供了线索。

项目成果

PriA binds to oriC segments upon induction of fork stall in Escherichia coli cells

在大肠杆菌细胞中诱导叉失速后 PriA 与 oriC 片段结合

• 发表时间: 2013
• 作者: Tanaka;T. and Masai;H.
• 通讯作者: H.

Detection of chromosomal fragile sites : application of specific PriA binding to arrested replication forks in living cells

染色体脆弱位点的检测：应用特异性 PriA 结合到活细胞中停滞的复制叉

• 发表时间: 2011
• 作者: Tanaka;T. and Masai;H.
• 通讯作者: H.

Detection of chromosomal fragile sites: application of specific PriA binding to arrested replication forks in living cells.

染色体脆弱位点的检测：应用特异性 PriA 结合到活细胞中停滞的复制叉。

• 发表时间: 2011
• 作者: 田中 卓;正井 久雄
• 通讯作者: 正井 久雄

Toward the elucidation of molecular basis of kinase activation and substrate recognition of Cdc7-Dbf4 kinase complex : use of Microsporidia as a model

阐明 Cdc7-Dbf4 激酶复合物激酶激活和底物识别的分子基础：使用微孢子虫作为模型

• 发表时间: 2012
• 作者: Iwasaki;R.;Tanaka;T. Shimada;A.;Kohda;D.;and Masai;H.
• 通讯作者: H.