Chromatin regulation by DNA replication and recombination

染色质结构影响DNA重组的路径选择。我们发现，着丝粒中形成的染色质结构阻止了单链退火(SSA)和交叉的发生。着丝粒特异的重组调控对于防止着丝粒的大染色体重排(GCR)非常重要。在真核生物中，许多前复制复合体(PreRCs)组装在每条染色体上。我们发现，前RCs的形成不仅对DNA复制本身很重要，而且对DNA双链断裂(DSB)的修复和着丝粒染色质结构的构建也很重要，可能是通过组蛋白乙酰化实现的。

Chromatin structures affect the pathway choice of DNA recombination. We found that the chromatin structures formed in centromere prevents single-strand annealing (SSA) and crossing over to take place. This centromere-specific regulation of recombination is important to prevent gross chromosomal rearrangement (GCR) in centromere.Numbers of pre-replicative complexes (preRCs) are assembled on each chromosome in eukaryotes. We found that the formation of preRCs is important not only for DNA replication per se but also for the repair of DNA double-strand breaks (DSBs) and for the construction of chromatin structures in centromere probably through histone acetylation.