Analysis of clubroot resistant genes and development of differential lines in Brassica rapa.

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Plasmodiophora brassicae which causes clubroot disease has been classified into four different pathotypes (G1 - G4). Chinese cabbage F1 cultivar, CR Shinki having CRb which is one of resistance genes, showed resistant to G3 and G4. We constructed a fine map of CRb locus using 2,032 F2 plants and developed new selective DNA markers. The position of CRb was narrowed to about 140kb between two new markers, KB59N07 and B1005. KiE7 (Brassica rapa) harboring another resistant gene, CRk, was resistance to P2,P3,P4. Using Brassica rapa DNA database, new DNA markers linked to CRk were developed. These DNA markers also linked to Crr3, another clubroot resistant gene. We cleared that the relationship between three resistance genes (CRb, CRk amd Crr3) and the pathotypes of clubroot fungus.