Biosynthesis mechanism of a cyclic antibacterial peptide linked at N- and C- terminal amino acids and elucidation of the cyclization significance
Biosynthesis mechanism of a cyclic antibacterial peptide linked at N- and C- terminal amino acids and elucidation of the cyclization significance
批准号:
23580101
负责人:
KAWAI Yasushi
金额:
$3.49万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2013
中文摘要
鉴定了由58个氨基酸残基组成的N-端和C-端连接的环状抗菌肽(细菌素)Gassericin A的生物合成基因,基因表达分析表明,前导序列对Gassericin A的合成起重要作用,而不是环化作用。此外,还推测Gassericin A通过ABC转运蛋白连接在N端和C端的氨基酸上,然后被一种可能的新型蛋白水解酶切割后分泌到生产细胞外。
英文摘要
A biosynthesis genes for gassericin A, which was the cyclic antibacterial peptide (bacteriocin) linked at N- and C-termini consisting of 58 amino acid residue, were identified, and gene expression analysis clarified that the leader sequence was important to production of gassericin A not the cyclization. In addition, it was suggested gassericin A was linked at N- and C-terminal amino acids by an ABC-transporter and then secreted to outside the producer cells after cleavage of the leader peptide by a putative novel proteinase.
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Lactobacillus gasseri用の安価な食品グレード培地の創製‐最適食品添加用酵母エキスの選抜‐
为加氏乳杆菌创建廉价的食品级培养基 - 选择食品添加剂的最佳酵母提取物 -
DOI:
--
发表时间:
2014
期刊:
影响因子:
--
作者:
[春日元気, 松島裕樹, 吉田光希, 荒川健佑, 川井泰, 宮本拓, 増田哲也]
通讯作者:
増田哲也
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[齋藤忠夫, 伊藤裕之, 岩附慧二, 吉岡俊満編, 井沢真吾, Yasushi Kawai and Tadao Saito, 井沢真吾分担執筆, Yasushi Kawai and Tadao Saito]
通讯作者:
Yasushi Kawai and Tadao Saito
ヨーグルトの事典・抗菌(性)物質
酸奶百科/抗菌物质
DOI:
--
发表时间:
2014
期刊:
影响因子:
--
作者:
[A. Iwaki, S. Ohnuki, Y. Suga, S. Izawa, and Y. Ohya, 川井泰]
通讯作者:
川井泰
ヨーグルトの事典
酸奶百科全书
DOI:
--
发表时间:
2014
期刊:
影响因子:
--
作者:
[齋藤忠夫, 伊藤裕之, 岩附慧二, 吉岡俊満編]
通讯作者:
吉岡俊満編
腸内細菌により生産されるバクテリオシン(抗菌性ペプチド・タンパク質)の最新事情:~構造、機能から近未来の利用性まで~
肠道细菌产生的细菌素(抗菌肽和蛋白质)的最新信息:〜从结构和功能到未来用途〜
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[中村圭志, 荒川健佑, 鈴木はるか, 安田成美, 川井泰, 伊藤喜之, 西村順子, 北澤春樹, 齋藤忠夫, 川井 泰]
通讯作者:
川井 泰
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