The analysis of the shift from gastric to intestinal phenotype of stem cell in the stomach mucosa

胃粘膜干细胞从胃向肠表型转变的分析

• 批准号: 23590921
• 负责人: MIZOSHITA Tsutomu
• 金额: $ 3.33万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590921/
• 关键词: 胃幹細胞; 三次元培養系

We established a long-term three-dimensional primary culture of mouse glandular stomach epithelial cells, being cultivated for more than 2 months and maintaining the gastric phenotype without intestinal one. We consider that the shift from gastric to intestinal phenotype of stem cell in the stomach mucosa may be clarified by applying the above-mentioned three-dimensional primary culture to the glandular stomach of Helicobacter pylori-infected Mongolian gerbil model in which the intestinal metaplasia often occurs.

我们建立了小鼠腺胃上皮细胞的长期三维原代培养，培养2个月以上，保持胃表型，无肠表型。我们认为，通过将上述三维原代培养应用于经常发生肠化生的幽门螺杆菌感染蒙古沙鼠模型的腺胃，可以阐明胃粘膜干细胞从胃到肠表型的转变。

项目成果

スナネズミはlansoprazole-associated collagenous colitis解析モデルとして有用である

沙鼠可用作兰索拉唑相关胶原性结肠炎分析的模型

• 发表时间: 2012
• 作者: 溝下勤;塚本宏延;谷田諭史;尾関啓司;城卓志
• 通讯作者: 城卓志

Adalimumabを投与したbiologic naive Crohn's disease症例の臨床的検討

阿达木单抗治疗克罗恩病生物学病例的临床研究

• 发表时间: 2014
• 作者: 溝下勤;谷田諭史;尾関啓司;塚本宏延;片野敬仁;林則之;田中守;濱野真吾;西脇裕高;海老正秀;澤田武;森義徳;久保田英嗣;片岡洋望;城卓志
• 通讯作者: 城卓志

Establishment of a long-term three-dimensional primary culture of mouse glandular stomach epithelial cells within the stem cell niche.

• DOI: 10.1016/j.bbrc.2013.02.051
• 发表时间: 2013-03-22
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Katano, Takahito;Ootani, Akifumi;Joh, Takashi
• 通讯作者: Joh, Takashi

Lansoprazole induces collagenous colitis in the colon of Mongolian gerbils.

• DOI: 10.1016/s0016-5085(11)62698-x
• 发表时间: 2011-05
• 期刊: Asian Pacific journal of cancer prevention : APJCP
• 作者: Takashi Mizushima;T. Mizoshita;M. Sasaki;S. Tanida;Hironobu Tsukamoto;T. Shimura;T. Kanematsu;H. Kataoka;T. Kamiya;T. Tsukamoto;M. Tatematsu;T. Joh

Long-term high-dose proton pump inhibitor administration to Helicobacter pylori-infected Mongolian gerbils enhances neuroendocrine tumor development in the glandular stomach.

• 发表时间: 2011
• 期刊: Asian Pacific journal of cancer prevention : APJCP
• 作者: Hironobu Tsukamoto;T. Mizoshita;M. Sasaki;Takashi Mizushima;S. Tanida;K. Ozeki;Yoshikazu Hirata;T. Shimura;H. Kataoka;T. Kamiya;S. Nojiri;T. Tsukamoto;M. Tatematsu;T. Joh