Molecular analyses on the intercellular signaling relating to the bone destruction of ameloblastoma

成釉细胞瘤骨质破坏相关细胞间信号传导的分子分析

• 批准号: 23592929
• 负责人: NAKAMURA Norifumi
• 金额: $ 3.33万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592929/
• 关键词: 口腔外科一般; エナメル上皮腫; 歯原性腫瘍; Wntシグナル; 歯原性上皮; 骨浸潤; 顎骨腫瘍; Wnt; MMP

Immortalized epithelial cell line derived from oral mucosa (MOE-1), as control, and immortalized ameloblastoma cell line derived from follicular-type ameloblastoma (AM-3) were established and their biological characteristics were compared to AM-1, previously established immortalized ameloblastoma cells derived from prexiform-type ameloblastoma.AM-1 and AM-3 demonstrated positive reaction to the epithelial markers and negative to the mesenchymal one maintaining the morphological characteristics of the epithelial cell. Expressions of MMP-2 and MMP-9 were significantly higher in AM-1 and AM-3 than MOE-1. Wnt-5a and Wnt signaling receptors, LRP and Frizzled, of both AM-1 and AM-3 were higher than MOE-1 in the mRNA level. Furthermore, expression and activity of MMP-9 in AM-3 increased dose- and time-dependently when stimulated with Wnt-3a protein.These results suggested that local invasiveness of ameloblastoma may be regulated by MMPs expression through the Wnt signaling pathway.

建立口腔黏膜上皮永生化细胞系（莫伊-1）和滤泡型成釉细胞瘤永生化细胞系（AM-3），并与AM-1进行生物学特性比较。先前建立的永生化成釉细胞瘤细胞来源于前型成釉细胞瘤AM-1和AM-2。3例上皮细胞标记阳性，间质细胞标记阴性，保持了上皮细胞的形态特征。MMP-2和MMP-9在AM-1和AM-3中的表达显著高于莫伊-1。AM-1和AM-3的Wnt-5a和Wnt信号受体LRP和Frizzled在mRNA水平均高于莫伊-1。Wnt-3a蛋白刺激AM-3细胞后，AM-3细胞MMP-9的表达和活性呈剂量和时间依赖性增加，提示MMP-9的表达可能通过Wnt信号通路调节成釉细胞瘤的局部侵袭性。

项目成果

新規口腔粘膜上皮細胞株M0E1の樹立-HPVやSV40を用いない不死化-

新型口腔粘膜上皮细胞系 M0E1 的建立 - 不使用 HPV 或 SV40 的永生化 -

• 发表时间: 2011
• 作者: 岐部俊郎;比地岡浩志;宮脇昭彦;中村典史;岸田昭世
• 通讯作者: 岸田昭世

The role of Wnt signaling pathway in growth characteristics of Ameloblastoma cells.

Wnt信号通路在成釉细胞瘤细胞生长特性中的作用。

• 发表时间: 2011
• 作者: Toshiro Kibe;Shosei Kishida;Hiroshi Hijioka;Akihiko Miyawaki;Norifumi Nakamura
• 通讯作者: Norifumi Nakamura

Establishment of novel ameloblastoma-derived cell line, and the role of Wnt signaling pathway in ameloblastoma.

新型成釉细胞瘤细胞系的建立及Wnt信号通路在成釉细胞瘤中的作用。

• 发表时间: 2013
• 期刊: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics
• 作者: Toshiro Kibe;Takao Fuchigami;Michiko Kishida;Mikio Iijima;Hiroshi Hijioka;Akihiko Miyawaki;Ichiro Senba;tooru Kiyono;Norifumi Nakamura;Shosei Kisida
• 通讯作者: Shosei Kisida

新規口腔粘膜上皮細胞株MOE1の樹立 -HPVやSV40遺伝子を用いない不死化-

新型口腔粘膜上皮细胞系MOE1的建立 - 不使用HPV或SV40基因的永生化 -

• 发表时间: 2011
• 作者: Toshiro Kibe;Shosei Kishida;Hiroshi Hijioka;Akihiko Miyawaki;Norifumi Nakamura;岐部俊郎，比地岡浩志，宮脇昭彦，中村典史，岸田昭世
• 通讯作者: 岐部俊郎，比地岡浩志，宮脇昭彦，中村典史，岸田昭世

Immortalization and characterization of MOE1 cells, normal oral epithelial cell lines without using HPV and SV40 genes-.

MOE1 细胞、正常口腔上皮细胞系的永生化和表征，无需使用 HPV 和 SV40 基因。

• 发表时间: 2011
• 期刊: Oral Science International
• 影响因子: 0.5
• 作者: Toshiro Kibe;Michiko Kidhida;Masayuki Kaimo,Mikiko Iiima;Lin Clen. Mika Habu;Akihiko;Miyawaki;Hiroshi Hijioka;Norifumi Nakamura;Tohru Kiyono;Shosei Kishida
• 通讯作者: Shosei Kishida