Molecular analyses on the intercellular signaling relating to the bone destruction of ameloblastoma

建立了来自口腔粘膜的永生化上皮细胞系（MOE-1）作为对照，以及来自滤泡型成釉细胞瘤的永生化成釉细胞瘤细胞系（AM-3），并将其生物学特性与AM-1（先前建立的来自预成形型成釉细胞瘤的永生化成釉细胞瘤细胞）进行了比较。AM-1和AM-3对上皮标记物呈阳性反应，对间质标记物呈阴性反应，维持上皮细胞的形态特征。MMP-2和MMP-9在AM-1和AM-3中的表达明显高于MOE-1。AM-1和AM-3的Wnt-5a及Wnt信号受体LRP和Frizzled mRNA水平均高于MOE-1。此外，在Wnt-3a蛋白刺激下，AM-3中MMP-9的表达和活性呈剂量和时间依赖性增加。这些结果提示，MMPs的表达可能通过Wnt信号通路调控成釉细胞瘤的局部侵袭性。

Immortalized epithelial cell line derived from oral mucosa (MOE-1), as control, and immortalized ameloblastoma cell line derived from follicular-type ameloblastoma (AM-3) were established and their biological characteristics were compared to AM-1, previously established immortalized ameloblastoma cells derived from prexiform-type ameloblastoma.AM-1 and AM-3 demonstrated positive reaction to the epithelial markers and negative to the mesenchymal one maintaining the morphological characteristics of the epithelial cell. Expressions of MMP-2 and MMP-9 were significantly higher in AM-1 and AM-3 than MOE-1. Wnt-5a and Wnt signaling receptors, LRP and Frizzled, of both AM-1 and AM-3 were higher than MOE-1 in the mRNA level. Furthermore, expression and activity of MMP-9 in AM-3 increased dose- and time-dependently when stimulated with Wnt-3a protein.These results suggested that local invasiveness of ameloblastoma may be regulated by MMPs expression through the Wnt signaling pathway.