Optically regulating the synaptic plasticity at the level of single synaptic spine.

在单个突触棘水平上光学调节突触可塑性。

• 批准号: 23650197
• 负责人: NAKAMURA Hideki
• 金额: $ 2.41万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650197/
• 关键词: 神経可塑性; ケミカルバイオロジー; 神経細胞生物学

As a preliminary step toward the establishment of technical methods to manipulate the synaptic transmission by rapamycin-induced protein dimerization paradigm, two plasmid libraries were constructed; PSD-95-based construct library and Rho GTPase family-based library. These libraries will contribute to the development of rapamycin-based methods to study synaptic plasticity in neuronal cells. To evaluate the rapamycin-induced protein dimerization within neurons, methods to monitor the diffusion kinetics of soluble proteins in the cell type were also established.

作为建立利用雷帕霉素诱导的蛋白二聚化模式操纵突触传递的技术方法的初步步骤，构建了两个质粒文库；基于psd -95的构造库和基于Rho GTPase家族的库。这些文库将有助于发展基于雷帕霉素的方法来研究神经元细胞的突触可塑性。为了评估雷帕霉素诱导的神经元内蛋白二聚化，还建立了监测细胞类型中可溶性蛋白扩散动力学的方法。

项目成果

Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier.

• DOI: 10.1038/nchembio.1252
• 发表时间: 2013-07
• 期刊: NATURE CHEMICAL BIOLOGY
• 影响因子: 14.8
• 作者: Lin, Yu-Chun;Niewiadomski, Pawel;Lin, Benjamin;Nakamura, Hideki;Phua, Siew Cheng;Jiao, John;Levchenko, Andre;Inoue, Takafumi;Rohatgi, Rajat;Inoue, Takanari
• 通讯作者: Inoue, Takanari

Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals.

• DOI: 10.1242/bio.20121081
• 发表时间: 2012-08-15
• 期刊: Biology open
• 影响因子: 2.4
• 作者: Narita K;Kozuka-Hata H;Nonami Y;Ao-Kondo H;Suzuki T;Nakamura H;Yamakawa K;Oyama M;Inoue T;Takeda S
• 通讯作者: Takeda S