Comprehensive analysis of mutant mammalian cells using barcode vector

我们以前开发了一种方法，用于快速分离大量的纯合突变小鼠ES细胞系。然而，我们意识到每个突变细胞系的表型分析是一个劳动密集型过程。在本研究中，我们构建了一个条形码载体文库，其中每个载体包含一个独特的核苷酸序列，并标记每个纯合突变细胞系与不同的条形码序列。这允许我们使用每个条形码序列作为代表相应突变细胞系的指标来评估每个突变细胞系在突变细胞库的整个群体中的比率，从而导致对合并细胞的快速表型分析。

We previously developed a method for rapid isolation of a large number of homozygous mutant mouse ES cell lines. However, we realized that phenotypic analysis of each mutant cell line is a labor-intensive process. In the current study, we have constructed a barcode vector library in which each vector contains a unique nucleotide sequence, and labeled each homozygous mutant cell line with different barcode sequence. This allowed us to evaluate the ratio of each mutant cell line among entire population of a pool of mutant cells using each barcode sequence as an index to represent the corresponding mutant cell line, leading to a rapid phenotypic analysis with pooled cells.