Development of vectors self-spreading into population by utilization of a homing endonuclease

利用归巢核酸内切酶开发自我扩散到群体中的载体

• 批准号: 23651205
• 负责人: JUNI Naoto
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651205/
• 关键词: 集団規模遺伝子操作; ベクター開発; 利己的遺伝子; ホーミングエンドヌクレアーゼ; ショウジョウバエ; 人工利己的遺伝子; ベクター; 集団規模の遺伝子操作; 人工利己的遺伝子ベクター; 集団的遺伝子操作

We attempted to develop a vector system self-expanding into insect populations that might be applicable to pest control in future. Self-expanding vectors should enable population-wide genetic manipulation by introducing a transgene into only a small fraction of population. To confer such property, we considered utilizing naturally occurring "selfish genetic elements", which gradually increase their own genetic frequency in population by generations. In this study, we designed and constructed several versions of model vectors comprising a homing endonuclease gene from yeast, which is one of the natural selfish genetic elements, introduced them into Drosophila and observed their behavior. Unfortunately, no successful self-expanding vector has been obtained so far.

我们试图开发一种自扩展到昆虫种群的媒介系统，可能适用于未来的害虫防治。通过将转基因引入一小部分种群，自扩展载体应该能够实现全种群的遗传操作。为了赋予这种特性，我们考虑利用自然发生的“自私遗传元素”，它们在种群中逐渐增加自己的遗传频率。在本研究中，我们设计并构建了几种包含天然自私遗传因子之一的酵母归巢内切酶基因的模型载体，并将它们引入果蝇并观察它们的行为。不幸的是，到目前为止还没有获得成功的自展开向量。