Innovative regenerative medicine of hearing loss after cochlear ischemia by embryonic stem cells

我们研究了沙土鼠耳蜗短暂缺血后HSC对进行性毛细胞变性的影响。通过颅外阻断双侧椎动脉造成短暂性耳蜗缺血。经股静脉给予HSC。螺旋蜗轴动脉中证实了移植细胞，但在Corti器官中未发现移植细胞。听力的顺序变化进行了评估ABR。HSCs组ABR阈移较对照组明显降低。HSC组内毛细胞的死亡/完整细胞的平均比率显著小于对照组。HSCs组Corti器中营养因子蛋白水平升高，说明HSCs上调了营养因子的表达。静脉注射造血干细胞可防止毛细胞变性，改善听力障碍。这些结果表明，HSC可能通过旁分泌作用具有治疗潜力。

We investigated the influence of HSCs on progressive hair cell degeneration after transient cochlear ischemia in gerbils. Transient cochlear ischemia was produced by extracranial occlusion of the bilateral vertebral arteries. HSCs were administrated via femoral vein. Transplanted cells were confirmed in the spiral modiolar artery but was not found in the organ of Corti. Sequential changes in hearing were assessed by ABR. HSCs group was significantly decreased ABR threshold shift compared to the control group. The average ratio of dead/intact cells of inner hair cells was significantly smaller in the HSCs group than control group. The increasing of trophic factors protein level in the organ of Corti in the HSCs group indicated that HSCs upregulated the trophic factors. The intravenous injected HSCs could prevent hair cell degeneration and ameliorate hearing impairment. These results suggested that HSCs had therapeutic potential possibly through paracrine effects.