The development of novel colorectal cancer screening method using exosomal antisense RNA in serum

利用血清中外泌体反义RNA筛查结直肠癌的新方法的开发

• 批准号: 23790613
• 负责人: CHIBA Mitsuru
• 金额: $ 2.83万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790613/
• 关键词: 腫瘍検査学; アンチセンス RNA; エキソソーム; RNA; 大腸癌; アンチセンスRNA; スクリーニング

Exosomes are microvesicles that are released from various cells into the extracellular space. It has been reported that the components within exosomes vary according to the type of secreted cell. In the present study, we identified a species of mRNAs, microRNAs, and natural antisense RNAs within the exosomes secreted from the three colorectal cancer (CRC) cell lines. We discovered that their natural antisense RNAs were within exosomes for the first time in the present study. Currently, the searching of exosomal CRC-specific natural antisense RNAs in serum has been performed.

外来体是从各种细胞释放到细胞外空间的微泡。据报道，外泌体内的组分根据分泌细胞的类型而变化。在本研究中，我们鉴定了三种结直肠癌（CRC）细胞系分泌的外泌体中的一种mRNA、microRNA和天然反义RNA。我们在本研究中首次发现它们的天然反义RNA存在于外泌体中。目前，已经在血清中进行了外泌体CRC特异性天然反义RNA的搜索。

项目成果

An efficient method for high-fidelity messenger RNA amplification from a small amount of total RNA.

一种从少量总 RNA 中进行高保真信使 RNA 扩增的有效方法。

• DOI: 10.3892/br.2012.15
• 发表时间: 2013
• 期刊: Biomedical Reports
• 影响因子: 2.3
• 作者: Kaneko;M.;Kanno;N.;Tanaka;R.;Suzuki;A.;and Yatomi;Y.;Mitsuru Chiba
• 通讯作者: Mitsuru Chiba