Role of Matrix Gla Protein in vascular calcification

基质 Gla 蛋白在血管钙化中的作用

• 批准号: 23790871
• 负责人: SUGANO Yasuo
• 金额: $ 2.66万
• 依托单位: National Cardiovascular Center Research Institute (2012-2013)International University of Health and Welfare (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790871/
• 关键词: 血管石灰化; マトリックス蛋白; 石灰化; 心血管疾患; トランスレーショナルリサーチ

No difference was observed between transgenic and wild-type mice in mortality, phenotypic characteristics (heart, lung, aorta, kidney, liver) as well as pathologic property at 6 weeks after birth. In addition, expression of mRNA and protein levels of GLA were not significantly increased in produced mice.The conclusion was that transgene expression was inactivated in vivo, although they were expressed in vitro.The promoter was switched to Tie-2, which also provide vascular-specific transgene expression. However, there were some difficulty in detecting extracellular expression of Gla protein, despite ordinary expression of intracellar extract. Special unexpected processing and binding to other proteins in cells might explain this phenomenon.

出生后6周，转基因小鼠和野生型小鼠的死亡率、表型特征（心、肺、主动脉、肾、肝）以及病理学特征均无差异。另外，GLA的mRNA和蛋白表达水平在产生的小鼠中没有显著增加。结论是，转基因表达在体内是失活的，尽管它们在体外表达。启动子被切换到Tie-2，这也提供了血管特异性转基因表达。然而，尽管细胞内提取物表达正常，但在检测Gla蛋白的细胞外表达方面存在一些困难。特殊的意想不到的处理和结合到细胞中的其他蛋白质可能解释这种现象。