Identification of Human facilitating cells

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We sought human facilitating cells using human / mouse xenon-transplantation model.Among human bone marrow CD8 positive cells, 30〜40% cells were positive for CD56 and 15 〜20% cells were CD161 positive. Bone marrow cells were sorted into CD8+CD56+, CD8+ CD56-, CD8+CD161+and CD8+CD161-using FACS Vantage as candidates for facilitating cells. Total bone marrow cells, CD34+c-kit+cells(stem cells), CD8+cells candidates for facilitating cells and stem cells plus candidates for facilitating cells were, respectively, transplanted into 300 cGy irradiated NOD / SCID mice. After 8 weeks in the transplantation, mice were sacrificed and the bone marrow cells were measured for human CD45 positive cells. Percentages of CD45 positive cells were 1 〜7% after total bone marrow cell transplantation, 3〜10% after stem cell transplantation, 0% after CD8 positive cell transplantation and candidates for facilitating cells, 3〜18% after stem cells plus candidates for facilitating cells transplantation. No significant differences were detected among the four candidates for facilitating cells plus candidates for facilitating cells transplantation. The low engrafting efficacy of the xenotransplantation was thought to cause the negative date, we then choose new born NOD / SCID β2microglobulin-/-mice as recipients. The engrafting efficacy was improved and over 90% of human CD45 positive cells were seen after total bone marrow cells transplantation. The study is still on going using the new xenon transplantation model.We have studied neutropenia of infancy and several new information were reported.