Screening of low molecular compounds, which inhibit cell proliferation and survival

筛选抑制细胞增殖和存活的低分子化合物

• 批准号: 23659488
• 负责人: KANAKURA Yuzuru
• 金额: $ 2.33万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659488/
• 关键词: 低分子化合物; 増殖; 細胞周期; 細胞株; 細胞増殖; アポトーシス; スクリーニング; 増殖抑制

We tried to establish a new anti-proliferative drug through a following process: (1) screening of compounds to inhibit Baf3 cell growth (2) identification of target molecules for the selected compounds (3) analysis of physical effects of each possible compound. We screened approximately 10,000 low molecular compounds, which were provided from Tokyo University, and successfully isolated 61 compounds. Some inhibited Baf3 cell growth, or induced apoptosis, and the others induced morphological changes. Among them, we selected 4 compounds, T-167317, T-170633, T-005170, T-051702, which is water-soluble and has a specific structure. To identify their molecular targets, we just fixed the compounds onto FG beads.

我们试图通过以下过程建立一种新的抗增殖药物：（1）筛选抑制Baf3细胞生长的化合物（2）鉴定所选化合物的靶分子（3）分析每种可能化合物的物理作用。我们筛选了东京大学提供的约 10,000 种低分子化合物，并成功分离出 61 种化合物。一些抑制 Baf3 细胞生长或诱导细胞凋亡，另一些则诱导形态变化。其中，我们选择了4个化合物，T-167317、T-170633、T-005170、T-051702，它们是水溶性的且具有特定的结构。为了确定它们的分子靶标，我们将化合物固定到 FG 珠上。

项目成果

Identification of osteoblast stimulating factor-5 as a novel regulator of early lymphocyte development.

鉴定成骨细胞刺激因子 5 作为早期淋巴细胞发育的新型调节剂。

• 发表时间: 2012
• 作者: Fujita N;et al
• 通讯作者: et al

Significance of novel HSC marker ESAM expression in cord blood

脐带血中新型 HSC 标志物 ESAM 表达的意义

• 作者: Fresquet V;et al.;T Ishibashi
• 通讯作者: T Ishibashi

Advances in Hematopoietic Stem Cell Research

造血干细胞研究进展

• 发表时间: 2012
• 作者: Yokota T;Oritani K;Butz S;Ewers S;Vestweber D;Kanakura Y.
• 通讯作者: Kanakura Y.

The endothelial antigen ESAM monitors hematopoietic stem cell status between dormancy and self-renewal

内皮抗原 ESAM 监测造血干细胞在休眠和自我更新之间的状态

• 发表时间: 2012
• 作者: Koarada S;Tada Y;Sohma Y;Haruta Y;Suematsu R;Mitamura M;Inoue H;Ehara H;Tokoro Y;Ohta A;Nagasawa K;Sudo T
• 通讯作者: Sudo T

The endothelial antigen ESAM level distinguishes HSC status between quiescence and self-renewal

内皮抗原 ESAM 水平区分 HSC 静止状态和自我更新状态

• 作者: Sudo T;et al
• 通讯作者: et al