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Challenges of visualizing the conformational changes of prestin using an atomic force microscope equipped with a membrane potential control device

Challenges of visualizing the conformational changes of prestin using an atomic force microscope equipped with a membrane potential control device
使用配备膜电位控制装置的原子力显微镜观察 prestin 构象变化的挑战
批准号:
23659790
负责人:
WADA Hiroshi
金额:
$2.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
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中文摘要
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英文摘要
A membrane potential control device, which induces the conformational changes of prestin expressed in the plasma membrane, has been newly developed. Microstructures and electrophysiological properties of the prestin-expressing culture cells and the prestin-reconstituted lipid bilayer were measured by using this device. As a result, it was suggested that the conformational changes of proteins in the plasma membrane may possibly be visualized when the membrane potential is controlled using the developed device. Based on the findings obtained in this study, further research to visualize the conformational changes of prestin reconstituted in the lipid bilayer will be performed using this device.
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Analysis of membrane topology of prestin expressing in CHO cells
CHO 细胞中表达的 prestin 的膜拓扑分析
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [Murakoshi M, Kawase T, Kumano S, Wada H]
通讯作者: Wada H
High‐Resolution AFM Imaging of Prestin Purified and Reconstituted into an Artificial Lipid Bilayer
Prestin 纯化并重构为人工脂质双层的高分辨率 AFM 成像
DOI: 10.1063/1.3658084
发表时间: 2011
期刊:
影响因子: --
作者: [H. Wada, S. Kumano, M. Murakoshi, Koji Iida, H. Hamana]
通讯作者: H. Hamana
DOI: --
发表时间: 2013
期刊:
影响因子: --
作者: [Ogawa Y, Murakoshi M, Wada H]
通讯作者: Wada H
DOI: --
发表时间: 2013
期刊:
影响因子: --
作者: [Tetsuo Maruyama, Akiko Yamasaki, Kaoru Miyazaki, Toru Arase, Hiroshi Uchida, Yasunori Yoshimura, 小川雄大,村越道生,和田仁]
通讯作者: 小川雄大,村越道生,和田仁
7
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