Challenges of visualizing the conformational changes of prestin using an atomic force microscope equipped with a membrane potential control device

使用配备膜电位控制装置的原子力显微镜观察 prestin 构象变化的挑战

• 批准号: 23659790
• 负责人: WADA Hiroshi
• 金额: $ 2.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659790/
• 关键词: 耳科学

A membrane potential control device, which induces the conformational changes of prestin expressed in the plasma membrane, has been newly developed. Microstructures and electrophysiological properties of the prestin-expressing culture cells and the prestin-reconstituted lipid bilayer were measured by using this device. As a result, it was suggested that the conformational changes of proteins in the plasma membrane may possibly be visualized when the membrane potential is controlled using the developed device. Based on the findings obtained in this study, further research to visualize the conformational changes of prestin reconstituted in the lipid bilayer will be performed using this device.

新近研制出一种膜电位控制装置，可诱导prestin在质膜上表达的构象变化。利用该装置对表达prestin的培养细胞和重组prestin的脂质双分子层进行微观结构和电生理特性的检测。结果表明，当利用该装置控制膜电位时，可以直观地看到质膜中蛋白质的构象变化。在本研究的基础上，我们将利用该装置进一步研究重组的prestin在脂质双分子层中的构象变化。

项目成果

Analysis of membrane topology of prestin expressing in CHO cells

CHO 细胞中表达的 prestin 的膜拓扑分析

• 发表时间: 2011
• 作者: Murakoshi M;Kawase T;Kumano S;Wada H
• 通讯作者: Wada H

High‐Resolution AFM Imaging of Prestin Purified and Reconstituted into an Artificial Lipid Bilayer

Prestin 纯化并重构为人工脂质双层的高分辨率 AFM 成像

• DOI: 10.1063/1.3658084
• 发表时间: 2011
• 作者: H. Wada;S. Kumano;M. Murakoshi;Koji Iida;H. Hamana
• 通讯作者: H. Hamana

Evaluation of electrophysiological properties of the motor protein prestin expressed in the inner ear

内耳表达的运动蛋白 prestin 的电生理特性评估

• 发表时间: 2013
• 作者: Ogawa Y;Murakoshi M;Wada H
• 通讯作者: Wada H

内耳に発現する膜タンパク質プレスチンの電気生理学的特性計測のための平面パッチクランプシステムの開発（Development of a planar patch clamp system for measuring electrophysiological properties of a membrane protein prestin expressed in the inner ear）

开发平面膜片钳系统，用于测量内耳中表达的膜蛋白 prestin 的电生理特性

• 发表时间: 2013
• 作者: Tetsuo Maruyama;Akiko Yamasaki;Kaoru Miyazaki;Toru Arase;Hiroshi Uchida;Yasunori Yoshimura;小川雄大，村越道生，和田仁
• 通讯作者: 小川雄大，村越道生，和田仁

内耳に発 現する膜タンパク質プレスチンの電気 生理学的特性計測のための平面パッチ クランプシステムの開発(Development of a planar patch clamp system for measuring electrophysiological properties of a membrane protein prestin expressed in the inner ear)

开发平面膜片钳系统，用于测量内耳中表达的膜蛋白 prestin 的电生理特性

• 发表时间: 2013
• 作者: Ikuko Kishi;Tetsuo Maruyama;Miho Ohishi;Ysuo Akiba;Hironori Asada;Yasuhiro Konishi;Masao. Nakano;Kiyoshi Kamei;Yasunori Yoshimra;小川雄大,村越道生,和田仁
• 通讯作者: 小川雄大,村越道生,和田仁