Challenges of visualizing the conformational changes of prestin using an atomic force microscope equipped with a membrane potential control device
Challenges of visualizing the conformational changes of prestin using an atomic force microscope equipped with a membrane potential control device
批准号:
23659790
负责人:
WADA Hiroshi
金额:
$2.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
关键词:
中文摘要
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英文摘要
A membrane potential control device, which induces the conformational changes of prestin expressed in the plasma membrane, has been newly developed. Microstructures and electrophysiological properties of the prestin-expressing culture cells and the prestin-reconstituted lipid bilayer were measured by using this device. As a result, it was suggested that the conformational changes of proteins in the plasma membrane may possibly be visualized when the membrane potential is controlled using the developed device. Based on the findings obtained in this study, further research to visualize the conformational changes of prestin reconstituted in the lipid bilayer will be performed using this device.
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Analysis of membrane topology of prestin expressing in CHO cells
CHO 细胞中表达的 prestin 的膜拓扑分析
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Murakoshi M, Kawase T, Kumano S, Wada H]
通讯作者:
Wada H
High‐Resolution AFM Imaging of Prestin Purified and Reconstituted into an Artificial Lipid Bilayer
Prestin 纯化并重构为人工脂质双层的高分辨率 AFM 成像
DOI:
10.1063/1.3658084
发表时间:
2011
期刊:
影响因子:
--
作者:
[H. Wada, S. Kumano, M. Murakoshi, Koji Iida, H. Hamana]
通讯作者:
H. Hamana
Evaluation of electrophysiological properties of the motor protein prestin expressed in the inner ear
内耳表达的运动蛋白 prestin 的电生理特性评估
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Ogawa Y, Murakoshi M, Wada H]
通讯作者:
Wada H
内耳に発現する膜タンパク質プレスチンの電気生理学的特性計測のための平面パッチクランプシステムの開発(Development of a planar patch clamp system for measuring electrophysiological properties of a membrane protein prestin expressed in the inner ear)
开发平面膜片钳系统,用于测量内耳中表达的膜蛋白 prestin 的电生理特性
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Tetsuo Maruyama, Akiko Yamasaki, Kaoru Miyazaki, Toru Arase, Hiroshi Uchida, Yasunori Yoshimura, 小川雄大,村越道生,和田仁]
通讯作者:
小川雄大,村越道生,和田仁
内耳に発 現する膜タンパク質プレスチンの電気 生理学的特性計測のための平面パッチ クランプシステムの開発(Development of a planar patch clamp system for measuring electrophysiological properties of a membrane protein prestin expressed in the inner ear)
开发平面膜片钳系统,用于测量内耳中表达的膜蛋白 prestin 的电生理特性
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Ikuko Kishi, Tetsuo Maruyama, Miho Ohishi, Ysuo Akiba, Hironori Asada, Yasuhiro Konishi, Masao. Nakano, Kiyoshi Kamei, Yasunori Yoshimra, 小川雄大,村越道生,和田仁]
通讯作者:
小川雄大,村越道生,和田仁
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