Study of a novel insulin signaling network mediated by GAPDH in H9c2 cardiac cell line

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In the rat cardiomyocyte-derived cell line H9c2 it has been shown that phosphorylation of GAPDH,one of the glycolytic enzymes, was induced by insulin treatment. In this study, GAPDH nitration and its association with actin were both induced by insulin treatment before the GAPDH phosphorylationresponse to insulin stimulation in the H9c2 cell line. Moreover, after treatment of the cell line with insulin, accumulation of GAPDH protein in the nucleus was observed. We also demonstrated the possibility that insulin signaling mediated by GAPDH was impaired in the heart of type 2 diabetic rat. These results suggest that GAPDH may play a key role in insulin signal transduction in cardiac muscle.