Elucidation of insecticide detoxification by hydrolases and evaluation of species difference between humans and experimental animals

水解酶对杀虫剂解毒作用的阐明以及人类与实验动物物种差异的评估

• 批准号: 23710042
• 负责人: NISHI Kosuke
• 金额: $ 2.75万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710042/
• 关键词: 健康影響評価; エステラーゼ; 殺虫剤; ピレスロイド; 加水分解; 種差

A library of recombinant hydrolases expressed in human liver was generated using the baculovirus expression system. When the library was screened with pyrethroids and their derivatives containing a fluorochrome, novel enzymes that hydrolyze the insecticides were discovered. Biochemical characteristics of the enzymes was analyzed.

利用杆状病毒表达系统生成了人肝脏中表达的重组水解酶文库。当用含有荧光染料的拟除虫菊酯及其衍生物对文库进行筛选时，发现了能水解这些杀虫剂的新型酶。分析了酶的生化特性。

项目成果

Studies on immunotoxicity of o-phenylphenol (OPP) and metabolite

邻苯基苯酚（OPP）及其代谢物的免疫毒性研究

• 发表时间: 2012
• 作者: Sugahara T;Nakata Y;Nishi K;Nishimoto S
• 通讯作者: Nishimoto S

Immunostimulatory in Vitro and in Vivo Effects of a Water-Soluble Extract from Kale

• DOI: 10.1271/bbb.100490
• 发表时间: 2011-01-01
• 期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
• 影响因子: 1.6
• 作者: Nishi, Kosuke;Kondo, Ai;Sugahara, Takuya
• 通讯作者: Sugahara, Takuya

Enzyme-Linked Immunosorbent Assay with Monoclonal and Single-Chain Variable Fragment Antibodies Selective to Coplanar Polychlorinated Biphenyls

• DOI: 10.1021/jf203585b
• 发表时间: 2012-02-22
• 期刊: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
• 影响因子: 6.1
• 作者: Inui, Hideyuki;Takeuchi, Tetsuya;Ohkawa, Hideo
• 通讯作者: Ohkawa, Hideo

Biologically active ester derivatives as potent inhibitors of the soluble epoxide hydrolase.

• DOI: 10.1016/j.bmcl.2012.07.074
• 发表时间: 2012-09-15
• 期刊: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
• 影响因子: 2.7
• 作者: Kim, In-Hae;Nishi, Kosuke;Kasagami, Takeo;Morisseau, Christophe;Liu, Jun-Yan;Tsai, Hsing-Ju;Hammock, Bruce D.
• 通讯作者: Hammock, Bruce D.

Immunomodulatory effects of citrus fruit auraptene in vitro and in vivo

柑橘类水果 auraptene 的体外和体内免疫调节作用

• DOI: 10.1016/j.jff.2012.06.005
• 发表时间: 2012
• 期刊: Journal of Functional Foods
• 影响因子: 5.6
• 作者: Daifuku M;Nishi K;Okamoto T;Nakano H;Nishimoto S;Sugahara T;Kota Kanda et al.;Agus Budiawan Naro Putra et al.;Kosuke Nishi et al.;In-Hae Kim et al.;Sogo Nishimoto et al.
• 通讯作者: Sogo Nishimoto et al.