Effect of intra-articular injection of MCP-1 on arthritis

关节内注射MCP-1对关节炎的影响

• 批准号: 24790995
• 负责人: FUJII Hiroshi
• 金额: $ 2.75万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790995/
• 关键词: リウマチ学; ケモカイン; 関節炎; 関節リウマチ

Previously, we reported that ablation of an inflammatory chemokine receptor, CCR2, led to aggravation of the arthritis of IL-1ra-/- mouse. We assume that MCP-1, the ligand of CCR2, may resolve the arthritis in IL-1ra-/- and injected repeatedly 100pg of MCP-1 into the joint of IL-1ra-/- mouse. Unexpectedly, more severe arthritis was observed after injection of MCP-1, accompanying severe accumulation of neutrophils and macrophages into the joint. Multiplex analysis of cytokine levels in the joint of mature IL-1ra-/-CCR2-/-mouse (20-week-old) revealed decreased levels of regulatory cytokines, such as IL-4 and IL-10, indicating regulatory roles of macrophages during late stage of arthritis. Collectively, CCR2+ macrophages may serve as pro-inflammatory during early stage and later, as anti-inflammatory during late stage of arthritis.

以前，我们报道了炎症趋化因子受体CCR 2的消融导致IL-1 ra-/-小鼠关节炎的加重。我们推测趋化因子受体2的配体MCP-1可以缓解IL-1 ra-/-小鼠的关节炎，并在IL-1 ra-/-小鼠关节内重复注射100 pg MCP-1。出乎意料的是，在注射MCP-1后观察到更严重的关节炎，伴随着中性粒细胞和巨噬细胞在关节中的严重积累。成熟IL-1 ra-/-CCR 2-/-小鼠（20周龄）关节中细胞因子水平的多重分析显示调节性细胞因子（如IL-4和IL-10）水平降低，表明巨噬细胞在关节炎晚期的调节作用。总的来说，CCR 2+巨噬细胞可以在关节炎的早期和晚期充当促炎剂，在关节炎的晚期充当抗炎剂。

项目成果

アダリムマブ投与有効例, 無効例, 再発例における血中サイトカイン濃度の変化

阿达木单抗有效、无效和复发时血液细胞因子浓度的变化

• 发表时间: 2014
• 作者: 藤井 博;藤澤 雄平;北田 欽也;鈴木 康倫;伊藤 清亮;水島 伊知郎;濱野 良子;山田 和徳;山岸 正和;川野 充弘
• 通讯作者: 川野 充弘