Analysis of regulation of nuclear receptor-mediated signal transdidian by the ligand-specific transporters
Analysis of regulation of nuclear receptor-mediated signal transdidian by the ligand-specific transporters
批准号:
18570104
负责人:
FUJII Hiroshi
金额:
$2.63万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2006
资助国家:
日本
项目状态:
已结题
起止时间:
2006 至 2007
中文摘要
为了阐明配体特异性转运蛋白在核受体(FXR:法尼醇X受体或PPAR:过氧化物酶体增殖物激活受体)介导的信号转导中的功能,我们试图鉴定和分析由PPAR或FXR介导的新型信号转导的分子机制。核受体FXR介导的信号转导分析:在研究过程中,我们发现胆汁酸转运蛋白(FABP 6/I-BABP,IBAT)和FXR共定位于大鼠卵巢。这些基因在卵巢分化过程中表达。此外,我们已经证明人OSBa/β基因被FXR和核受体LXR(肝X受体)上调(Pharmaceutical Res.24:390-398,2007)。核受体PPAR/FXR介导的信号转导在癌发生或转移中的分析:我们发现核受体PPAR靶基因FABP-5(C-FABP:皮肤脂肪酸结合蛋白)在人类恶性前列腺癌或乳腺癌中上调,并参与这些肿瘤的转移。FABP-5通过其siRNA的下调引起癌症转移发生率的降低(Mt.肿瘤学杂志32:767-775,2008)。此外,我们已经证明FABP 6在人结肠直肠癌中上调(Clin. Cancer Res.12(17):5090-5095,2006)。新型PPAR介导的信号转导的分析:我们发现FABP 5和FABP 7(B-FABP)在大鼠脑中的生长锥中特异性表达,这对神经变性和再生过程很重要。我们已经为每个FABP引入了siRNA,以分析它们在生长锥中的功能。此外,我们还尝试使用免疫沉淀分析来检测与FABP 5或FABP 7相互作用的特异性蛋白质。
英文摘要
To elucidate function of ligand-specific transporters in the nuclear receptor (FXR: farnesoid X receptor or PPAR: peroxysome proliferator-activated receptor)-mediated signal transduction, we have tried to identify and analyze molecular mechanisms of a novel signal transduction mediated by PPAR or FXR.1. Analysis of nuclear receptor FXR-mediated signal transduction:During the course of studies, we have revealed that bile acid transporters (FABP6/I-BABP, IBAT) and FXR are co-localized in the rat ovary. These genes are expressed during the ovary differentiation. Furthermore, we have demonstrated that human OSBa/β gene is up-regulated by FXR and nuclear receptor LXR (liver X receptor) (Pharmaceutical Res. 24:390-398, 2007)2. Analysis of nuclear receptors PPAR/FXR-mediated signal transduction in carcinogenesis or metastasis:We have found that the nuclear receptor PPAR target gene, FABP-5 (C-FABP: cutaneous fatty acid-binding protein), is up-regulated in human malignant prostate or breast cancers and that is involved in metastasis in these tumors. Down-regulation of FABP-5 by its siRNA caused decrease in the incidence of cancer metastasis (Mt. J. Oncol. 32:767-775, 2008). Furthermore, we have demonstrated that FABP6 is up-regulated in human colorectal cancers (Clin. Cancer Res. 12 (17):5090-5095, 2006).3. Analysis of novel PPAR-mediated signal transduction:We have found that FABP5 and FABP7 (B-FABP) are specifically expressed in the growth cone in the rat brain important for the neurodegeneration and regeneration process. We have introduced siRNA for each FABP to analyze their functions in the growth cone. Furthermore, we have tried to examine specific proteins interacting with FABP5 or FABP7 using the immunoprecipitation assay.
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血中腸型脂肪酸結合蛋白測定による急性腸炎診断
血液肠脂肪酸结合蛋白测定诊断急性肠炎
DOI:
--
发表时间:
2007
期刊:
影响因子:
--
作者:
[]
通讯作者:
DOI:
10.1093/ndt/gfn139
发表时间:
2008-08-01
期刊:
NEPHROLOGY DIALYSIS TRANSPLANTATION
影响因子:
6.1
作者:
[Kimura, Hideki, Li, Xuan, Yoshida, Haruyoshi]
通讯作者:
Yoshida, Haruyoshi
Establishment and characterization of monoclonal and polyclonal antibodies against human intestinal fatty acid-binding protein(I-FABP).
抗人肠脂肪酸结合蛋白(I-FABP)单克隆和多克隆抗体的建立和表征。
DOI:
--
发表时间:
2008
期刊:
J. Immunoassay & Immunochem 29
影响因子:
--
作者:
[H.L. Wong, R. Pinontoan, K. Hayashi, R. Tabata, T. Yaeno, K. Hasegawa, C. Kojima, H. Yoshioka, K. Iba, T. Kawasaki, K. Shimamoto, Y. Saito, H. L. Wong, Y.Tanaka, M.Mishima, Y.Tanaka, K.Furuita, 児嶋長次郎, H. Kimura, E. A. Morgan, S. Kajiura]
通讯作者:
S. Kajiura
Expression of cutaneous fatty acid-binding protein (C-FABP) in prostate cancer: Potential prognostic marker and target for tumourigenecity-suppression
皮肤脂肪酸结合蛋白(C-FABP)在前列腺癌中的表达:潜在的预后标志物和肿瘤发生抑制的靶点
DOI:
--
发表时间:
2008
期刊:
Int. J. Oncol. 32
影响因子:
--
作者:
[H.L. Wong, R. Pinontoan, K. Hayashi, R. Tabata, T. Yaeno, K. Hasegawa, C. Kojima, H. Yoshioka, K. Iba, T. Kawasaki, K. Shimamoto, Y. Saito, H. L. Wong, Y.Tanaka, M.Mishima, Y.Tanaka, K.Furuita, 児嶋長次郎, H. Kimura, E. A. Morgan, S. Kajiura, E.A. Morgan, S. Kajiura, H. Kimura, E. A. Morgan]
通讯作者:
E. A. Morgan
Development and evaluation of a new sandwich ELISA system for intestinal fatty acid Binding protein (I-FABP) concentrations in human serum
开发和评估人血清中肠脂肪酸结合蛋白 (I-FABP) 浓度的新型夹心 ELISA 系统
DOI:
--
发表时间:
2007
期刊:
Jpn. J. of Med. & Parmceut. Sci 58, 5
影响因子:
--
作者:
[H.L. Wong, R. Pinontoan, K. Hayashi, R. Tabata, T. Yaeno, K. Hasegawa, C. Kojima, H. Yoshioka, K. Iba, T. Kawasaki, K. Shimamoto, Y. Saito, H. L. Wong, Y.Tanaka, M.Mishima, Y.Tanaka, K.Furuita, 児嶋長次郎, H. Kimura, E. A. Morgan, S. Kajiura, E.A. Morgan, S. Kajiura, H. Kimura, E. A. Morgan, S. Kajiura, X. Li, M. Okuwaki, 舟岡 宏幸, X. Li, M. Okuwaki, H. Funaoka]
通讯作者:
H. Funaoka
共 25 条
Studies on estimation method of key transcription factors by bioinformatics
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批准号:16K14857
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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负责人:FUJII Hiroshi
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Development of the crops diagnosis system using the newly developed digital camera
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Effect of intra-articular injection of MCP-1 on arthritis
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批准号:24790995
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Development of genetic statistics verification base in effect of breeding based on allele analysis
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批准号:23580055
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Molecular Mechanism Controlling Reactivity and Selectivity of High-Valent Oxo-Metal Complexes
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Top-down mechanisms of perception: dynamical-systems scenarios based on neurophysiology
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Functional analysis of fatty acid-binding proteins (FABPs) involved in nuclear receptor signaling
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批准号:22570112
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Functional analysis of autophagy in T cells of healthy donors and SLE patients
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Transitions between "internal states" in the brain viewed as quasi-attractors- A mathematical and neurodynamical approach
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Inhibitory Background Field Hypothesis Generated by Gap Junction-Coupled Interneuron Systems in the Neocortex
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Synthesis and Characterization of High Valnet Reaction Intermediate of Non-Heme Iron Enzymes
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Studies on Novel Nuclear Receptor-mediated Signal Transduction
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Functional Alteration of Myoglobin to Nitrite Reductase by Synthetic Heme and Protein Mutations
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批准号:12640549
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项目类别:Grant-in-Aid for Scientific Research (C)
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STUDY ON PHYSIOLOGICAL FUNCTION OF CHYMOTRYPSIN INHIBITORS IN BOMBYX MORI
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资助金额:$26.27万
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负责人:FUJII Hiroshi
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依托单位:
Mathematical Study of the Dynamical Cell Assembly Hypothesis as the Encoding Principle of the Brain
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批准号:10640140
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项目类别:Grant-in-Aid for Scientific Research (C)
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财政年份:1998
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负责人:FUJII Hiroshi
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依托单位:
Development of Anti-loosening Screw Fasteners
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批准号:10450064
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$3.07万
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财政年份:1998
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负责人:FUJII Hiroshi
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依托单位:
Integration of linkage map, RFLP map and chromosomal map
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批准号:09045069
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$4.03万
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财政年份:1997
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依托单位:
A Theoretical and Empirical Study on the Mechanism of BOJ's Market Operation
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批准号:09630095
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Studies on Genetic Resources of the silkworm, Bombyx mori
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批准号:08456037
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Mathematical Study of The Dynamical cell Assembly Hypothesis as the Encoding mechanism of the brain
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.28万
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财政年份:1996
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负责人:FUJII Hiroshi
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依托单位:
国内基金
海外基金
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基于FCER1G基因介导免疫反应探讨迟发性聋与认知障碍相关性的机制研究
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