Studies on Genetic Resources of the silkworm, Bombyx mori

发现了新的突变体、胭脂和不蜕皮。通过连锁分析将ro基因定位在性染色体上。测定胰凝乳酶抑制剂13 （CI-13）的核苷酸序列。具有纯合子ki基因的成虫产肾形卵，不能发育完整的胚胎。这些结果表明，ki卵的母体mrna与正常卵的母体mrna不同。利用从正常和ki突变体的未受精卵中提取的mrna，采用RT-PCR和差异显示方法来阐明它们之间的差异。结果表明，ki突变体的母源mRNA与正常突变体的mRNA无明显差异，而隐性突变体“soft and limp”的sol基因与位于第6连锁组末端的E基因有连锁。E^<ca>与位于同一连锁群上的F基因的三点检验表明，三个基因位点的排列顺序为sol-E^<ca>-F，确定sol基因的位点为第6连锁群的-21.1 cM。为了澄清这一结果，用家蚕触角基因的RNA探针进行了原位杂交，荧光信号在染色体的内部区域被检测到，而不是在其远端。

New mutants, rouge and non-molting were found. The ro gene was located on the sex chromosome by linkage analysis. Nucleotide sequence of chymotrypsin inhibitor 13 (CI-13) was determined.The adult having homozygous ki gene lays kidney-shaped eggs which can not develop complete embryo. These results suggest that maternal mRNAs of the ki egg differ from maternal mRNAs of the normal egg. Using the mRNAs extracted from unfertilized eggs of a normal and ki mutant, RT-PCR and differential display methods was performed to elucidate the difference between them. The results indicated that maternal mRNA from ki mutant are not different from that of a normal..The sol gene for a recessive mutant named "soft and limp" was shown to be linked with E gene mapped to the end of the 6th linkage group. A three-point test involving the E^<ca> and the F gene located on the same linkage group indicated that the arrangement of three gene loci is in the order of sol-E^<ca>-F The locus of the sol gene was determined to be -21.1 cM of the 6th linkage group. In oeder to clarify this result, in situ hybridization with a RNA probe derived from the Bombyx mori Antennapedia gene was carried out, the fluorescence signal was detected at an internal region of a chromosome, not at its distal end.