Studies on Genetic Resources of the silkworm, Bombyx mori

通过连锁分析，将ro基因定位于性染色体上。测定了糜蛋白酶抑制剂13（CI-13）的核苷酸序列，ki基因纯合的成虫产肾形卵，不能发育成完整的胚胎。这些结果表明ki卵的母体mRNA不同于正常卵的母体mRNA。利用RT-PCR和差异显示技术，从正常和ki突变体的未受精卵中提取mRNA，以阐明它们之间的差异。结果表明，ki突变体的母体mRNA与正常对照无明显差异。一个隐性突变体sol基因与E基因连锁，位于第6连锁群的末端。对<ca>位于同一连锁群上的E^和F基因的三点检验表明，三个基因位点的排列顺序为sol-E^<ca>-F，其中sol基因位于第6连锁群的-21.1cM处。为了阐明这一结果，用来源于家蚕幼虫足基因的RNA探针进行原位杂交，在染色体的内部区域检测到荧光信号，而不是在其远端。

New mutants, rouge and non-molting were found. The ro gene was located on the sex chromosome by linkage analysis. Nucleotide sequence of chymotrypsin inhibitor 13 (CI-13) was determined.The adult having homozygous ki gene lays kidney-shaped eggs which can not develop complete embryo. These results suggest that maternal mRNAs of the ki egg differ from maternal mRNAs of the normal egg. Using the mRNAs extracted from unfertilized eggs of a normal and ki mutant, RT-PCR and differential display methods was performed to elucidate the difference between them. The results indicated that maternal mRNA from ki mutant are not different from that of a normal..The sol gene for a recessive mutant named "soft and limp" was shown to be linked with E gene mapped to the end of the 6th linkage group. A three-point test involving the E^<ca> and the F gene located on the same linkage group indicated that the arrangement of three gene loci is in the order of sol-E^<ca>-F The locus of the sol gene was determined to be -21.1 cM of the 6th linkage group. In oeder to clarify this result, in situ hybridization with a RNA probe derived from the Bombyx mori Antennapedia gene was carried out, the fluorescence signal was detected at an internal region of a chromosome, not at its distal end.