Functional analysis of fatty acid-binding proteins (FABPs) involved in nuclear receptor signaling

参与核受体信号传导的脂肪酸结合蛋白 (FABP) 的功能分析

• 批准号: 22570112
• 负责人: FUJII Hiroshi
• 金额: $ 2.58万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570112/
• 关键词: 核内受容体; 脂肪酸結合タンパク質; 脂肪酸; 胆汁酸; シグナル伝達; 遺伝子発現; 癌転移; miRNA; マイクロRNA

(1) Functional analysis of FABP5 in nuclear receptor PPAR signaling in carcinogenesis or metastasis: I have found that nuclear receptor PPAR target gene, FABP5, is up-regulated in human malignant prostate cancer cellsand that is localized into the nucleus in a ligand (fatty acid)-dependent manner.(2)Functional analysis of FABP6 in nuclear receptor FXR signaling: I have identified novel miRNAs and their target genes that were activated by the FXR agonist. Furthermore, I have demonstrated that a variant protein for FABP6, called FABP6-L/IBABP-L, has exhibited anti-apoptotic activity in the presence of bile acid in colorectal cancer cells.(3) Activation mechanisms of FABP5 gene expression: I have found that DNA methylation status of FABP5 gene isolated from PC-3 is much more demethylated compared withthat of the gene from PNT-2.

(1)FABP 5在核受体PPAR信号通路中的功能分析：我发现核受体PPAR靶基因FABP 5在人恶性前列腺癌细胞中表达上调，并以配体（脂肪酸）依赖的方式定位于细胞核。（2）FABP 6在核受体FXR信号传导中的功能分析：我已经鉴定了新的miRNAs及其被FXR激动剂激活的靶基因。此外，我已经证明了FABP 6的变体蛋白，称为FABP 6-L/IBABP-L，在结肠直肠癌细胞中存在胆汁酸的情况下表现出抗凋亡活性。(3)FABP 5基因表达的激活机制：我发现从PC-3分离的FABP 5基因的DNA甲基化状态比从PNT-2分离的基因的DNA甲基化状态更去甲基化。

项目成果

青色光刺激を与えてキノコ菌糸の代謝経路を制御する

蓝光刺激控制蘑菇菌丝的代谢途径

• 发表时间: 2012
• 作者: 小嶋 政信;藤井 博
• 通讯作者: 藤井 博

Analysis of the mechanism of prostatic cancer metastasis mediated by human epidermal-type fatty scid-binding protein (C-FABP/FABP5)

人表皮型脂肪scid结合蛋白(C-FABP/FABP5)介导的前列腺癌转移机制分析

• 发表时间: 2011
• 作者: 川口耕一郎;藤井博
• 通讯作者: 藤井博

Makabe Synthesis of procyanidin C2 & C1 using Lewis acid mediated equimolar condensation.

Makabe 原花青素 C2 的合成

• 发表时间: 2012
• 期刊: Heterocycles
• 影响因子: 0.6
• 作者: Y. Oizumi;M. Katoh;Y. Hattori;K. Toda;K. Kawaguchi;H. Fujii;and H.
• 通讯作者: and H.

Hatakeyama Diagnosis of ischemic small bowel disease by measurement of serum intestinal fatty acid-binding protein in patients with acute abdomen: a multicenter, observer-blinded validation study.

Hatakeyama 通过测量急腹症患者血清肠脂肪酸结合蛋白诊断缺血性小肠疾病：一项多中心、观察者盲法验证研究。

• 发表时间: 2011
• 期刊: J. Gastroenterology
• 作者: T. Kanda;A. Tsukahara;K. Ueki;Y. Sakai;T. Tani;A. Nishimura;T. Yamazaki;Y. Tamiya;T. Tada;M. Hirota;J. Hasegawa;H. Funaoka;H. Fujii;K.
• 通讯作者: K.

Development of a high-specificity sandwich ELISA system for the quantificaion of human intestinal fatty acid-binding protein(I-FABP)concentrations.

开发高特异性夹心 ELISA 系统，用于定量人肠脂肪酸结合蛋白 (I-FABP) 浓度。

• 发表时间: 2011
• 期刊: Immunological Investigation
• 作者: H.Funaoka;TKanda;S.Kajiura;Y.Ohkaru;H.Fujii
• 通讯作者: H.Fujii