Integration of linkage map, RFLP map and chromosomal map

本研究的目的是综合经典链路图、RFLP链路图和细胞学图。我们发现了一个新的牡蛎突变体，并从遗传上分析了它，突变体是由一个被称为受体的基因（软）和被命名的晶体控制的。通过使用bl和Se的三点实验，软基因位于15个链接组的42.7。一些变种人，罗（红眼斑点）， gr-r（recessive gray egg），Ki-2（kidney），Ly D1ca D1（Caltrop-type Multilunar）及索尔（软和limp 9是经遗传分析的，属于第一链接组（性链接）， gr-r在7， 5在第二链接组，ki-2到19链接组，李伊D1ca李伊D1 to 4 th linkage group and sol at 21.0 on 6 th linkage group. RFLP linkage analysis using the probes from embryonic cDNA library was carried out。我们揭示了13个探测器属于每个独立的连接组：1 st（性染色体），第四（L），第五（L）（E-D1）、7 th（g）、11 th（K）、13 th（ch）、14 th（U）、15 th（bl）、19 th（nb）、20 th（oh）、21 st（Lan）、22 nd（or）、和第24（出售）用FISH （原位杂交）用pFib2.9探针（纤维哈维链基因属于第25链接组）， Fib-H被观察到定位在染色体的末端，其位置在第25染色体上未知。FISH被携带出使用性连接的巧克力（sch）基因作为探针，在第一个链接组中检测到一个荧光信号，这表明FISH在21.3位置良好的结果，该结果表明FISH被放置在第一个链接组中。

The purpose of this research is to integrate classical linkage map, RFLP linkage map and cytological map. We obtained a new oily mutant and analyzed genetically it. The mutant was controlled by a recessive gene (oft) and named translucent-15. By three point experiment using bl and Se, the oft gene was located at 42.7 on the 15 linkage group. Some mutants, ro (red eye spot), gr-r (recessive gray egg), ki-2 (kidney), LィイD1caィエD1 (Caltrop-type multilunar) and sol (soft and limp9 were analyzed genetically, ro belonged to 1st linkage group (sex linked), gr-r at 7, 5 on 2nd linkage group, ki-2 to 19th linkage group, LィイD1caィエD1 to 4th linkage group and sol at 21.0 on 6th linkage group.RFLP linkage analysis using the probes from embryonic cDNA library was carried out. We revealed that 13 probes belonged to each independent linkage group : 1st (sex chromosome), 4th (L),5th (EィイD1ElィエD1), 7th (g), 11th (K), 13th (ch), 14th (U), 15th (bl), 19th (nb), 20th (oh), 21st (Lan), 22nd (or), and 24th (Sel)By FISH (in situ hybridization) using pFib2.9 probe (Fibroin Havey chain gene belongs to the 25th linkage group), Fib-H was observed to be located at an end of chromosome, although its locus was unknown on the 25th chromosome. FISH was carried out using sex-linked chocolate (sch) gene as a probe, fluorescent signal was detected on one third of an chromosome, it indicated that the result of FISH consisted well with the sch locus located at 21.3 on the 1st linkage group.