Investigation on the mechanisms of skin residual deneritic cells in the development psoriasis vulgaris.

皮肤残留树突状细胞在寻常型银屑病发生发展中的机制探讨

• 批准号: 24791189
• 负责人: YOSHIKI Ryutaro
• 金额: $ 2.66万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791189/
• 关键词: 尋常性乾癬; ランゲルハンス細胞; ガンマデルタＴ細胞; Ｔｈ１７; IL-23; IL-17; インターロイキン２３; ガンマデルタT細胞; インターロイキン１７; IL-12/23p40; gamma-delta T細胞

Psoriasis is a common chronic inflammatory skin disease that involves dysregulated interplay between immune cells and keratinocytes. Langerhans cells (LCs) are a subset of dendritic cells that reside in the epidermis and regulate immune responses. In this study, we focused on Th17-related factors and assessed the role of LCs and gamma-delta T cells in the development of psoriasis using a mouse psoriasis model triggered by topical application of imiquimod (IMQ). Langerhans cell depletion by means of diphtheria toxin (DT) in Langerin DT receptor-knocked-in mice suppressed hyperkeratosis, parakeratosis and ear swelling in the IMQ-treated regions. In addition, LC-depleted mice showed decreased levels of Th17-related cytokines in IMQ-treated skin lesions. These results suggest that LCs are required for the development of psoriasis-like lesions induced by IMQ in mice.

银屑病是一种常见的慢性炎症性皮肤病，涉及免疫细胞和角质形成细胞之间的相互作用失调。朗格汉斯细胞（LC）是树突状细胞的一个子集，位于表皮中并调节免疫反应。在这项研究中，我们专注于Th 17相关因子，并评估了LC和γ-δ T细胞在银屑病发展中的作用，使用由局部应用咪喹莫特（IMQ）引发的小鼠银屑病模型。在Langerin DT受体敲入小鼠中，通过白喉毒素（DT）消除朗格汉斯细胞抑制了IMQ处理区域的角化过度、角化不全和耳肿胀。此外，LC耗竭小鼠在IMQ治疗的皮肤病变中显示出Th 17相关细胞因子水平降低。这些结果表明，LCs是由IMQ诱导的小鼠银屑病样病变的发展所必需的。

项目成果

IL-23 from Langerhans cells is Required for the Development of Imiquimod-Induced Psoriasis-Like Dermatitis by Induction of IL-17A-Producing gamma-delta T cells

通过诱导产生 IL-17A 的 γ-δ T 细胞，产生咪喹莫特诱导的银屑病样皮炎需要来自朗格汉斯细胞的 IL-23

• 发表时间: 2014
• 期刊: Journal of Investigative Dermatology
• 影响因子: 6.5
• 作者: Yoshiki R;Kabashima K;Honda T;Nakamizo S;Sawada Y;Sugita K;Yoshioka H;Ohmori S;Malissen B;Tokura Y and Nakamura M
• 通讯作者: Tokura Y and Nakamura M

Langerhans cells are required for imiquimod-induced psoriasiform skin inflammation in mice.

朗格汉斯细胞是咪喹莫特诱导的小鼠银屑病皮肤炎症所必需的。

• 发表时间: 2012
• 作者: Yoshiki R;Kabashima K;Honda T;Nakamizo S;Sawada Y;Sugita K;Yoshioka H;Ohmori S;Malissen B;Tokura Y and Nakamura M;Ryutaro Yoshiki;吉木 竜太郎
• 通讯作者: 吉木 竜太郎