Researches in enzymes which digest allergic proteins of soybean

大豆过敏蛋白消化酶的研究

• 批准号: 24700810
• 负责人: MASAKI Takeshi
• 金额: $ 2.83万
• 依托单位: Nagoya University of Arts and Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700810/
• 关键词: アレルギー・ぜんそく; 蛋白質; 酵素; 植物; 食品; ダイズ; 発芽; プロテアーゼ; ダイズ（大豆）; 種子貯蔵タンパク質; アレルゲン; 酵素タンパク質

We revealed that three major allergic proteins (alpha-subunit of beta-conglycinin, Gly m Bd 28K, Gly m Bd 30K) of soybean decrease remarkably under soybean seed germination or soybean seedling growth. Although alpha-subunit of beta-conglycinin and Gly m Bd 28K have disappeared until 5days after imbibition, Gly m Bd 30K started to decrease since 14days after imbibition. It shows that Gly m Bd 30 K is degraded by another protease from that degrade alpha-subunit of beta-conglycinin and Gly m Bd 28K. In addition, Gly m Bd 28K in solution prepared from soybean seedlings decreased when its solution was incubated. Such in vitro decrease of Gly m Bd 28 K was disturbed by addition of aspartic protease inhibitor. It indicates that some kind of aspartic protease degrade Gly m Bd 28K.

我们发现，大豆的三种主要过敏蛋白（β-伴大豆球蛋白的α亚基、Gly m Bd 28K、Gly m Bd 30K）在大豆种子萌发或大豆幼苗生长过程中显着减少。虽然β-伴大豆球蛋白的α亚基和Gly m Bd 28K直到吸液后5天才消失，但Gly m Bd 30K从吸液后14天开始减少。它表明Gly m Bd 30 K被另一种蛋白酶降解，该蛋白酶降解β-伴大豆球蛋白和Gly m Bd 28K的α亚基。此外，大豆苗制备的溶液中的Gly m Bd 28K在其溶液孵育时降低。 Gly m Bd 28 K 的这种体外降低受到添加天冬氨酸蛋白酶抑制剂的干扰。这表明某种天冬氨酸蛋白酶降解Gly m Bd 28K。